气相色谱法同时测定艾叶中4种挥发性成分  

Simultaneous Determination of Four Volatile Components in Artemisia Argyi by Gas Chromatography

作　　者：陈桂琴[1] 李桂生[1] 罗香[1] 

机构地区：[1]江西省分析测试研究所,南昌330029

出　　处：《化学分析计量》2015年第4期44-47,共4页Chemical Analysis And Meterage

摘　　要：建立同时测定艾叶中桉油精、樟脑、薄荷脑、龙脑4种挥发性成分含量的分析方法。样品采用水蒸气蒸馏法提取。色谱柱采用DB-WAX毛细管柱（30m×0．32mm，0．25μm）；载气为氮气（99．99％），分流比为10：1；进样口温度为240℃；升温程序：柱温起始温度70℃，保持1min，然后以5℃／min的速率升温至150℃，保持2min，再以10℃／min的速率升温至200℃，保持6min；氢火焰离子化检测器（FID）温度为240℃。桉油精的质量浓度在0．05-0．8mg／mL范围内与色谱峰面积呈良好的线性，相关系数r〉0．999；樟脑、薄荷脑、龙脑的质量浓度在0．01—0.2mg／mL范围内与色谱峰面积线性关系良好，相关系数r〉0．999；方法回收率为92．00％-98．40％，测定结果的相对标准偏差为1．58％-2．44％（n=6）。桉油精、樟脑、薄荷脑、龙脑检出限分别为0．66，0．76，0．81，0．83mg／kg。该方法操作简单，准确度与灵敏度高，适合同时测定艾叶中桉油精、樟脑、薄荷脑、龙脑4个挥发性成分含量。A method was established for determinations of four volatile components （eucalyptol, camphor, menthol and borneol） in artemisia argyi. The target analytes of artemisia argyi were extracted by steam distillation, and separated by programmed temperature with Agilent DB-WAX capillary column（30 m×0.32mm,0.25μm）. The carrier gas was nitrogen（99.99%）, split ratio was 10 ： 1. The inlet temperature was 240 ℃. The column temperature program： the initial temperature was 70 ℃, maintained for 1 min, raised to 150 ℃ with rate of 5 ℃ /rain, maintained for 2 min, and at last raised to 200℃ with rate of 10℃/min, maintained for 6 min. The FID detector temperature was 240℃. The concentration of eucalyptol was linear with peak area in the range of 0.05-0.8 mg / mL. The concentration of camphor, menthol and bomeol was linear with peak area in the range of 0.01-0.2 mg/mL. The correlation coefficient was more than 0.999. The recovery rates ranged from 92.00% to 98.40%, with relative standard deviations of 1.58%-2.44%（n=6）. The detection limits of eucalyptol, camphor, menthol and borneol were 0.66,0.76,0.81,0.83 mg/kg, respectively. This method is simple to operate with high accuracy and sensitivity. It is suitable for simultaneous determination of four volatile components in artemisia argyi.

关 键 词：艾叶 气相色谱法 桉油精 樟脑 薄荷脑 龙脑 

分 类 号：O657.7[理学—分析化学]