白芷中10种呋喃型香豆素对长春新碱肠道转运的影响  

Effects of 10 furanocoumarins in Angelica dahurica on intestinal transport of vincristine

作　　者：管雪静 余世平[2] 廖正根[1] 朱卫丰[1] 赵国巍[1] 罗云[1] 梁新丽[1] 

机构地区：[1]江西中医药大学现代中药制剂教育部重点实验室,江西南昌330004 [2]江西中医药大学,南昌330004

出　　处：《中草药》2015年第14期2117-2121,共5页Chinese Traditional and Herbal Drugs

基　　金：国家青年自然科学基金项目(81303237);江西省卫生厅项目(2012A030);江西省青年科学基金项目(20114BAB15040);"赣鄱英才"工程领导人才培养计划(赣财教指[2013]296号)

摘　　要：目的研究自芷中10种吠喃型香豆素成分:氧化前胡素、佛手柑内酷、珊瑚菜素、欧前胡素、异欧前胡素、自当归脑、异补骨脂素、东蓑若内酷、伞形花内酷、自当归素对长春新碱吸收转运的影响。方法采用人源结肠癌细胞系Caco-2细胞单层模型,以长春新碱透过Caco-2细胞单层的表观渗透系数(papp)为指标,分别考察自芷中10种吠喃型香豆素类成分对长春新碱透过Caco-2细胞单层的影响。结果 10种香豆素类化介物对长春新碱转运的影响小同,氧化前胡素、异欧前胡素、欧前胡素能促进长春新碱透过Caco-2细胞单层转运(P<0.05);佛手柑内酷、自当归脑、异补骨脂素、伞形花内酷、自当归素对长春新碱透过Caco-2细胞单层转运有抑制作用(P<0.01);珊瑚菜素、东蓑若内酷对长春新碱透过Caco-2细胞单层转运无明显影响。结论小同种类吠喃型香豆素类化介物对长春新碱的肠道转运的影响小同,虽然均是吠喃型香豆素,对药物的转运可表现为完全相反的作用,可能与吠喃型香豆素类的结构以及其连接的官能团有关。Objective To study the effects of 10 kinds of furanocoumarines in Angelica dahurica （oxypeucedanin, isoimperatorin, phellopyerin, isoimperatorin, imperatorin, byakangelicol, angelicin, scopoletin, umbelliferone, and byakangelicine） on the intestinal absorption and transportation of vincristine. Methods The apparent permeability coefficient （Papp） of vincristin was used as evaluated parameter, and Caco-2 （human colon cancer cell lines） cell monolayer was used as intestinal epithelial cell model to study the effects of 10 furanocoumarins on transportation of vincristine in Caco-2 cell monolayer. Results There were different effects of the 10 furanocoumarins on transportation of vincristine. Oxypeucedanin, isoimperatorin, and imperatorin could improve the transport of vincristine in Caco-2 cell monolayer （P 〈 0.05）, while isoimperatorin, byakangelicol, angelicin, umbelliferone, and byakangelicine could inhibit the transport of vincristine in Caco-2 cell monolayer （P 〈 0.01）. However, phellopyerin and scopoletin have no effects on the transport of vincristine on Caco-2 cell. Conclusion Different kinds of furanocoumarins have different effects on intestinal transport of vincristine, which may be related to the structure of furocoumarins and the functional group about their connection.

关 键 词：白芷 呋喃型香豆素 长春新碱 Caco-2单层细胞 转运 

分 类 号：R285.5[医药卫生—中药学]