林麝AR基因外显子1、4、8的克隆和多态性检测  

Colone and Polymorphism Analysis of Exon1,Exon4 and Exon8in Androgen Receptor Gene of Muschus Berezorskii

作　　者：王永奇[1] 白康[2] 任战军[2] 刘文华[1] 李斐然[1] 黎勇 朱承嗣 唐婕[1] 唐青山 王方荣 

机构地区：[1]陕西省动物研究所,陕西西安7100321 [2]西北农林科技大学动物科技学院,陕西杨凌712100 [3]陕西镇坪逢春林麝养殖有限公司,陕西镇坪725600

出　　处：《家畜生态学报》2015年第6期11-14,25,共5页Journal of Domestic Animal Ecology

基　　金：陕西省科学院基础研究项目(2010K-09)

摘　　要：雄性激素受体(AR)基因外显子多态性可能与动物生产性能存在一定的相关关系。检测雄性林麝AR基因外显子1、4、8的多态性,探究其多态位点与泌香量的相关性,以期为选育高产优质麝香的林麝奠定基础。采用聚合酶链反应-单链构象多态性分析方法检测雄性林麝AR基因外显子1、4、8的多态性,并对其单核苷酸序列进行测序分析。结果发现:雄麝AR基因外显子1、4、8的扩增片段在雄麝中均不存在多态性。雄性林麝AR基因外显子1、4、8具高度保守性。Polymorphisms of Androgen Receptor（AR）gene exon are correlated with animal performance.To breed male Moschus berezovskii with high yield and quality of musk secretion,we detected the polymorphism of Exon1,Exon4 and Exon8in Androgen Receptor（AR）gene,and explored the relationship between polymorphic loci of AR gene and musk secretion quantity,utilized PCR-SSCP and cloning sequence in analyzing the polymorphism of Exon1,Exon4 and Exon8in AR gene of male Muschus berezorskii.Results showed that polymorphism of Exon1,Exon4 and Exon8in AR gene of male Muschus berezorskii does not exist.Exon1,Exon4 and Exon8in AR gene of male Muschus berezorskii were highly conserved.

关 键 词：林麝 雄性激素受体 PCR-SSCP 

分 类 号：S811.6[农业科学—畜牧学]