副猪嗜血杆菌感染禁食初乳仔猪实验动物模型的构建  

Establishment of Experimental Model for Colostrum-deprived Piglets Infected by Haemophilus Parasuis

作　　者：陈洪波[1] 程蕾 向敏 赵书红[3] 侯永清[1] 黎旺明 

机构地区：[1]武汉轻工大学动物科学与营养工程学院,动物营养与饲料安全湖北省协同创新中心,湖北武汉430023 [2]武汉市农科院畜牧兽医科学研究所,湖北武汉430208 [3]华中农业大学动物科学技术学院,农业动物遗传育种与繁殖教育部重点实验室,湖北武汉430070 [4]通城县种畜场,国家级华中两头乌猪通城猪保种场,湖北通城437400

出　　处：《家畜生态学报》2015年第6期53-62,共10页Journal of Domestic Animal Ecology

基　　金：国家自然科学基金项目(31301939);武汉市科技局应用基础研究计划项目(2013020501010178);湖北省自然科学基金项目(2013CFB324)

摘　　要：为了利用禁食初乳（colostrum-deprived,CD）仔猪构建可靠的猪格拉泽氏病实验动物模型,本研究分别选取12头新生通城仔猪和15头新生杜长大仔猪,母猪自然分娩后对新生仔猪立即隔离并做必要消毒处理后实施人工喂养;45～46日龄,CD仔猪经气管内接种致死剂量（109 CFU）的副猪嗜血杆菌（0165菌株,血清型5）。结果显示：⑴利用本研究优化、建立的饲喂方案使得CD仔猪攻毒前存活率达到80%～83.3%;⑵所有通城CD仔猪经攻毒后临床上表现出典型的格拉泽氏病病征,其中66.7%的CD仔猪可于攻毒后存活3d;而杜长大CD仔猪可能由于存在猪胸膜肺炎放线杆菌感染导致攻毒后无明显病征;⑶通城CD仔猪剖检后明显可见纤维素性心包炎、腹膜炎、关节炎等病症,并在血液和肺脏组织中成功鉴定到细菌;⑷组织病理分析发现,细菌感染导致心脏等多种组织存在纤维蛋白沉积伴随血管肿胀和血栓,脾脏组织淋巴细胞数量减少;⑸感染可能抑制淋巴细胞增殖并导致外周血中单核细胞数量减少。以上结果表明,本研究利用通城CD仔猪成功建立了副猪嗜血杆菌感染的实验动物模型;副猪嗜血杆菌和猪胸膜肺炎放线杆菌双阴性是保证模型构建成功的关键。To develop the reliable experimental model of Glsser＇s disease in colostrum-deprived（CD）piglets,a total of 12 newborn Tongcheng piglets and 15 newborn Duroc×Landrace×Large white piglets were selected after natural delivery.Piglets were collected immediately after birth and were artificially reared in a clean research facility.At the age of 45-46 d,CD piglets from both breeds were inoculated intratracheally（IT）with lethal does（109CFU）of Haemophilus Parasuis（HPS）serovar 5（strain 0165）.The results showed that：⑴ 80%-83.3% experimental piglets used in the CD survived until the infection date.⑵ Glsser＇s disease was successfully reproduced using Tongcheng CD piglets;In CD pigs a dose of109 infected 100% of the piglets and 66.7% of pigs was appropriate to allow the study of the disease up to3 days post inoculation.While none of the Duroc×Landrace×Large white CD pigs did not develop disease,probably due to Actinobacillus pleuropneumoniae（APP）infections.⑶ HPS-challenged Tongcheng pigs had inflammatory lesions compatible with Glsser＇s disease such as severe fibrinous polyserositis observed in the pericardial,pleural,and peritoneal cavities,characterized by the presence of fibrin strands.Also,HPS was isolated from lung and the peripheral blood.⑷ Histopathological examination found that HPS-challenged piglets presented a transudation of plasma proteins（fibrin deposits）in tissues such as brain combining with edema and vascular thrombosis;In addition,a lymphocyte reduction in spleen was found.⑸Investigation of the blood parameters suggested that HPS infection may inhibit the proliferation of lymphocytes and result in the reduction of monocyte in peripheral blood.In conclusion,a successful experimental model can be constructed on HPS infection using CD piglets;HPS and APP double negative pigs are critical for the reliability of challenge experiments.The present study prepared for the research in mechanisms of the HPS-host interactions and the porcine candidate gene ident

关 键 词：副猪嗜血杆菌 禁食初乳 感染模型 通城猪 

分 类 号：S811.6[农业科学—畜牧学]