成年大鼠许旺细胞复合去细胞神经支架修复坐骨神经缺损  

Repairment of sciatic nerve defect by Schwann cell of adult rat combined with acellular nerve grafts

作　　者：周丽娜[1] 崔晓军[1] 苏开鑫[1] 王晓红[1] 蔡雪彦[1] 郭金华[1] 纳青青[1] 

机构地区：[1]广东医学院解剖教研室,广东湛江524023

出　　处：《器官移植》2015年第4期268-272,共5页Organ Transplantation

基　　金：国家自然科学基金(81401022);湛江市科技公关计划项目(2012C3102012);广东省科技计划项目(2014A020211026)

摘　　要：目的探讨成年许旺细胞(SC)复合去细胞神经支架构建的组织工程化人工神经移植治疗外周神经损伤的效果。方法从Wallerian变性1周的成年SD大鼠远侧端神经中分离培养得到SC,复合去细胞神经支架构建组织工程化人工神经。移植分为SC+去细胞SD大鼠神经支架组(SC治疗组)和空细胞SD大鼠神经支架组(阴性对照组),每组各5只。比较两组大鼠术后2、4、8周损伤侧坐骨神经功能指数(SFI),术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率等修复效果的指标。结果 SC治疗组术后2、4、8周损伤侧SFI,术后8周损伤侧坐骨神经传导功能和小腿三头肌湿重恢复率均优于或高于阴性对照组(均为P<0.05)。结论采用成年SC复合去细胞神经支架的人工神经移植治疗外周神经缺损,可有效促进损伤神经的功能恢复。Objective To investigate clinical efficacy of tissue-engineered artificial nerve grafts constructed by acellular nerve grafts combined with adult rat Schwann cell（ SC） in the treatment of peripheral nerve injury. Methods SCs were isolated and cultured from the distal nerves of adult Sprague Dawley（ SD）rats with 1-week Wallerian degeneration and then combined with acellular nerve grafts to construct tissueengineered artificial nerve. All rats were divided into acellular nerve graft containing SCs（ SC group） and nerve graft containing no cells groups（ control group）,five animals in each group. At 2-,4- and 8-week after surgery,sciatic function index（ SFI） of the affected side was compared between two groups. At postoperative8 weeks,nerve conduction of sciatic nerve of the injured side,recovery rate of triceps surae wet weight and other relevant parameters were equally compared between two groups. Results In the SC group,SFI of the affected side at 2-,4- and 8-week after surgery,nerve conduction of sciatic nerve at the injured side and recovery rate of triceps surae wet weight at postoperative 8 weeks were significantly better compared with those in the control group（ all in P〈0. 05）. Conclusions Combined use of adult rat SCs and acellular nerve grafts effectively repairs peripheral nerve defects and accelerates functional recovery of injured nerves.

关 键 词：许旺细胞 移植 去细胞神经支架 神经再生 

分 类 号：R617[医药卫生—外科学]