柴苓汤对环孢素A肾病大鼠MCP-1、TNF-a的调控作用  

Regulation Effect of Chailing Decoction on MCP-1,TNF-a of the Cyclosporine A Aephropathy Rats

作　　者：黄晋红 潘秋霞[2] 张城浩 王香婷[2,3] 王聪慧[2] 魏民[3] 

机构地区：[1]武警河北总队医院,石家庄050081 [2]河北医科大学,石家庄050017 [3]河北中医学院,石家庄050200

出　　处：《中国中医基础医学杂志》2015年第7期812-815,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：河北省自然科学基金资助项目(H2014206284)

摘　　要：目的:观察柴苓汤对慢性环孢素A肾病大鼠单核细胞趋化蛋白-1(monocyte chemoattractant protein-1,MCP-1)及肿瘤坏死因子-a(tumour necrosis factor-a,TNF-a)的影响,探讨炎症反应在环孢素A肾病中的作用及柴苓汤的调控机制。方法:采用经口灌服环孢素A[30 mg/(kg·d)]的方法复制慢性环孢素A肾病大鼠模型,同时给予柴苓汤[3 g/(kg·d)]及缬沙坦[10 mg/(kg·d)]治疗共28 d,摘取肾脏观察肾脏病理变化。RT-PCR、免疫组化方法检测大鼠肾脏MCP-1、TNF-a蛋白及mRNA的表达。结果:病理显示模型组大鼠肾脏大量炎性细胞浸润,肾小管间质胶原成分明显增多,与对照组比较,MCP-I、TNF-a蛋白及mRNA表达显著增强。经柴苓汤及缬沙坦治疗,大鼠肾脏炎性细胞浸润及胶原沉积均减轻,MCP-I及TNF-a高表达被显著下调。结论:柴苓汤可减轻炎症损伤,减少ECM沉积,从而延缓慢性Cs A肾病纤维化进程。Objective： To observe the effect of Chailing Decoction（ CLD） on monocyte chemoattractant protein-1（ MCP-1） and tumour necrosis factor-α（ TNF-α） in rats with chronic cyclosporine A nephropathy（ CCN） for investigating the function of inflammation and regulatory mechanism of CLD. Methods： The CCN rat models were prepared using oral administration of cyclosporine A（ 30 mg·kg^- 1·d^- 1）. Meanwhile,they were treated with CLD（ 3 g·kg^- 1·d^- 1） and valsartan（ 10 m g·kg^- 1·d^- 1） by gastrogavage for 28 days respectively. The kidneys were harvested on the 29 th day and observed the pathology changes. The protein and gene expressions of MCP-I and TNF-α were observed using RT-PCR and immunohistochemical assay. Results： Renal pathology showed the inflammatory cell infiltration and collagen deposition in the renal interstitial area of Cs A group was significantly increased. Compared with the control group,the protein and mRNA expressions of MCP-I and TNF-α of Cs A group were significantly enhanced,and the high levels were down-regulated after treatment. Conclusions： CLD could retard the progress of CCN renal interstitial fibrosis by alleviating inflammation and reducing the deposition of ECM.

关 键 词：柴苓汤 环孢素A肾病 MCP-1 TNF-A 

分 类 号：R285.5[医药卫生—中药学]