DBBF修饰提高人脐带血红蛋白P_(50)的理化条件研究  

Effect of different reaction conditions on properties of DBBF-modified fetal hemoglobin products

作　　者：张学俊[1] 李燊[1] 周文涛[1] 陈刚[1] 刘嘉馨[1] 杨成民[1,2] 王红[1] 

机构地区：[1]中国医学科学院北京协和医学院输血研究所,四川成都610052 [2]天津协和生物科技发展研究所

出　　处：《中国输血杂志》2015年第6期627-630,共4页Chinese Journal of Blood Transfusion

基　　金：国家高技术研究发展计划(863计划)子课题(2012AA021903-3);四川省技术创新工程专项(2013ZZ0006);四川省国际科技合作与交流研究计划(2014HH0065)

摘　　要：目的提高人脐带血血红蛋白(Hb F)P50以使血红蛋白(Hb)能更好地向组织供氧。方法采用双(3,5-二溴水杨基)延胡索酸酯(DBBF)修饰人脐带血血红蛋白(Hb F)提高P50到血液生理值26 torr,以影响修饰反应溶液的pH、HbF浓度以及DBBF与Hb F反应的摩尔比为3个主要条件,血氧分析仪测定氧合曲线得到P50和希尔系数,按三者各自较佳反应条件试验得到DBBF修饰后HbF最优工艺,检测其所得样品P50、希尔系数及波尔效应等。结果Hb的底物浓度为7 g/d L,反应溶液p H为8.00,DBBF与HbF反应的摩尔比为2∶1,能提高HbF的P50接近血液生理值26 torr。结论筛选修饰工艺中反应溶液的pH、Hb浓度及DBBF与Hb F反应的摩尔比等3个条件,可使DBBF修饰提高HbF的P50,有助于修饰后HbF能更好地向组织供氧。Objective To improve the oxygen carrying capacity of Hb and to use a special human umbilical cord blood hemoglobin （HbF） modified by Bis（3,5-dibromosalicyl） fumarate （DBBF） to increase P50 to 26 torr. Methods DBBF was applied to modify HbF in order to increase P50 to blood physiological value on the three premises ： the pH of modified reaction solution, the concentration of HbF and the molar ratio of DBBF and HbF. From the oxygen dissociation/saturation curve, the Hill coefficient and P50 value were obtained, from which, their optimal reaction condition helped to determine the optimal HbF modified by DBBF process and the indices： P50 ,Hill CO and Bohr effect etc. Results The Hb＇s substrate concentration was 7.0 g/dl. The reaction solution＇s pH was 8.00. The ratio of DBBF and HbF was 2： 1. Besides, the modified HbF was more effective to improve the P50 value to 26 torr. Conclusion To explore the the modification process in reaction solution, including pH, Hb concentration and mole ratio in the reaction of DBBF and HbF, modified HbF is more effective to improve the P50 value, thus enhancing its ability to deliver oxygen to tissues and building a foundation for HBOC product development

关 键 词：人脐带血血红蛋白 双(3 5-二溴水杨基)延胡索酸酯 血红蛋白修饰 氧合曲线 50%氧分压(P50) 组织供氧 希尔系数 

分 类 号：R331.141[医药卫生—人体生理学] R457.7[医药卫生—基础医学]