泥鳅肝细胞的分离、纯化及TR——MTSEA透膜  

Isolation Purification and TR of Loach Hepatocytes by the Method of MTSEA Permeable Membrane

作　　者：张彬彬[1] 于敏[1] 闫苗苗[1] 王玖[1] 

机构地区：[1]滨州医学院生物科学实验中心,山东烟台264003

出　　处：《山东化工》2015年第14期120-121,123,共3页Shandong Chemical Industry

基　　金：滨州医学院科技计划项目;项目编号:BY2014KJ16

摘　　要：目的:以泥鳅为试验对象,通过分离、纯化其肝细胞以及TR——MTSEA透膜试验,为研究环境毒理学、细胞生理学、药理学等相关研究领域提供基础依据。方法:采用天然胶原酶H消化法分离纯化肝细胞。结果 :可成功得到含血细胞和其他杂细胞比较少的肝细胞。MTSEA为不能透过泥鳅肝细胞细胞膜的小分子氧化剂。结论 :用天然胶原酶H消化法分离纯化肝细胞,可提高肝实质细胞纯度。MTSEA作为不透膜的小分子氧化剂可用来研究更深层次的细胞调控。Objective To provide the basis for environmental toxicology, cell physiology, pharmacology and other related field of study, the trial was carried out by isolation , purification and TR of Loach hepatocytes by the method of MTSEA permeable membrane. Methods Loach hcpatocytes was isolated, purified by using natural collagenase H digestion method. Results： Blood cells and hepatocytes which contain other miscellaneous cells were obtained successfully. MTSEA is a small molecule oxidation agent that can not through the Loach hepatocytes membrane. Conclusions： Isolation purification and TR of Loach hepatocytes by using natural collagenase H digestion can improve the purity of the hepatic parenchyma cell. MTSEA as a small impermeable membrane molecule oxidation agent can be used for cell regulation deeper research.

关 键 词：泥鳅 肝脏 分离 纯化 MTSEA 

分 类 号：S961.6[农业科学—水产养殖]