乙胺嘧啶与氯喹降低感染疟疾的BALB/c孕小鼠的胎盘病理样改变  

Pyrimethamine and Chloroquine Reduced Malaria Infected Pregnant BALB/c Mice with Pathology Changes

作　　者：彭兰[1] 范能全 

机构地区：[1]重庆医药高等专科学校基础部,重庆400030 [2]重庆市食品药品检验所,重庆400042

出　　处：《南京师大学报（自然科学版）》2015年第2期105-111,共7页Journal of Nanjing Normal University(Natural Science Edition)

基　　金：重庆市高等教育教学改革项目(133272);重庆医药高等专科学校校级教研教改项目(ygzjg201304)

摘　　要：怀孕期间患疟疾与胎儿死亡、流产、早产及低体重密切相关,在一些疟疾发病地区,常常使用乙胺嘧啶和氯喹来预防治疗,但它们对于胎盘的病理样改变尚未报道,本研究的目的即为探讨乙胺嘧啶和氯喹治疗怀孕的BALB/c小鼠感染伯氏疟原虫诱发的疟疾引起胎盘的病理样改变.购买BALB/c小鼠,随机分为6组,建立疟疾模型,并使用乙胺嘧啶和氯喹治疗处理,对相应组的疟疾感染情况、小鼠生存率、氧化水平、凋亡水平及组织病理改变情况进行检测分析.结果发现,被伯氏疟原虫感染后血液中被感染的红细胞比例显著上升(p<0.05),同时小鼠存活率显著降低(p<0.05),而乙胺嘧啶和氯喹不但可以降低感染红细胞的比例,还能提升小鼠的存活率(p<0.05);被感染的小鼠的脂质过氧化水平和过氧化氢酶水平显著上升(p<0.05),使用乙胺嘧啶和氯喹治疗后又显著下降(p<0.05),而谷胱甘肽和超氧化物歧化酶的变化则不具有统计学差异(p>0.05);被感染的小鼠的凋亡细胞比例、感染红细胞形态和组织病理色素沉积较未感染组显著上升(p<0.05),使用乙胺嘧啶和氯喹治疗后又显著下降(p<0.05).乙胺嘧啶和氯喹在治疗疟疾中,通过抑制氧化应激及细胞凋亡的发生,降低胎盘组织病理学改变,从而达到有效的治疗目的.Malaria during pregnancy is closely related to the fetal death,miscarriage,premature delivery and low birth weight in some areas,which are often used to be prevent by pyrimethamine and chloroquine treatment,but the pathological changes in the placenta has not been reported in this study. The purpose is to explore pyrimethamine and chloroquine treatment of pregnant BALB / c mice infected with Plasmodium berghei malaria-induced pathology caused by placenta-like changes. BALB / c mice were randomly divided into 6 groups,the establishment of malaria model,and use of pyrimethamine and chloroquine therapy treatment for malaria infection in the corresponding group of mice survival,oxidation level,apoptosis and histopathological change the situation that were detected and analyzed. After being infected with Plasmodium berghei,the proportion of infected red blood cells was increased significantly（ p〈0. 05）,while the survival rate was significantly lower（ p〈0. 05）. Pyrimethamine and chloroquine can not only reduce the proportion of infected erythrocytes,but also enhance the survival rate of mice（ p〈0. 05）,lipids in mice infected hydrogen peroxide levels and oxidative enzyme levels increased significantly（ p0.05）,which was decreased after the treatment of pyrimethamine and chloroquine（ p0.05）,while changes in glutathione and superoxide dismutase was not a significant difference（ p0.05）,proportion of apoptotic cells in the infected mice,infected red blood cell morphology and pigmentation histopathology increased significantly compared with the uninfected group（ p0.05）,the use of pyrimethamine and chloroquine treatment and then decreased significantly（ p〈0. 05）. Pyrimethamine and chloroquine in the treatment of malaria by inhibiting oxidative stress and apoptosis occurs,reduce placental histopathological changes,and achieve effective therapeutic purposes.

关 键 词：疟疾 病理 乙胺嘧啶 氯喹 

分 类 号：R365[医药卫生—病理学]