靶向金纳米棒对隐球菌活性影响的体外实验研究  

Effect of targeted gold nanoparticles on viability of Cryptococcus neoformans in vitro research

作　　者：肖琴[1] 陈敏 徐楠[1] 

机构地区：[1]同济大学附属东方医院皮肤科,上海200120 [2]上海市长征医院皮肤科,上海200003 [3]上海市医学真菌分子生物学重点实验室,上海200003

出　　处：《中国真菌学杂志》2015年第3期134-138,共5页Chinese Journal of Mycology

基　　金：国家自然科学基金(81201234);上海市卫生局科研课题(2012143)

摘　　要：目的设计对隐球菌荚膜特异性标记的靶向金纳米棒,研究靶向金纳米棒的体外光热作用对隐球菌活性的影响。方法晶种生长法制备金纳米棒,偶联隐球菌荚膜抗体,检测表征,与隐球菌体外孵育,近红外激光照射,检测隐球菌活性变化。结果成功制备与荚膜抗体偶联的金纳米棒,体外近红外照射后,隐球菌活性较未偶联抗体的金纳米棒组显著降低。结论靶向性金纳米棒显著增强了近红外激光对隐球菌的光热效应,可用于治疗隐球菌感染的新尝试。Objective To design targeted gold nanoparticles（ GNP） conjugated with anti-Cryptococcus neoformans antibody,to explore targeted gold nanoparticles-mediated photothermal therapy of Cryptococcus neoformans in vitro. Methods Gold nanoparticles were synthesized according to seed-mediated template-assisted protocol,conjugated with anti-Cryptococcus neoformans antibody（ Ab）.Cryptococcus neoformans were incubated with targeted gold nanoparticles,then exposed to near-infrared（ NIR） light.Cell viability was evaluated.Results Targeted gold nanoparticles were synthesized successfully and can be used to selectively target Cryptococcus neoformans cells and following NIR laser exposure,can be used to significantly reduce cell viability.Conclusions These results demongstrated that Ab-mediated immunity gold nanoparticles can enhance NIR photothermal treatments effect on Cryptococcus neoformans.

关 键 词：隐球菌 金纳米棒 光热治疗 

分 类 号：R379.5[医药卫生—病原生物学]