特康唑对常见念珠菌的体外敏感性试验  

The sensitivity testing in vitro of major Candida to terconazole

作　　者：朱均昊[1] 韩德忞 李莉 刘健惠 朱小婵 章强强[1] 

机构地区：[1]复旦大学附属华山医院皮肤科真菌室,上海200040 [2]中国医药工业研究总院,上海200040

出　　处：《中国真菌学杂志》2015年第3期151-153,共3页Chinese Journal of Mycology

基　　金：国家科技重大新药创制(2012ZX09303004-001)

摘　　要：目的研究特康唑对重度念珠菌性阴道炎常见念珠菌的体外敏感性。方法菌株分离自上海交通大学医学院附属仁济医院、上海市第六人民医院、上海市第一妇婴保健院妇科门诊外阴阴道念珠菌病（VVC）患者的阴道分泌物。所有菌株均经念珠菌显色培养基（CHROMagar Candida）、API 20C AUX鉴定,并采用CLSIM27-A3肉汤微量稀释法对213株临床分离念珠菌作了特康唑、克霉唑、咪康唑3种抗真菌药物敏感性测定。结果 213株菌株经鉴定为白念珠菌192株（90.14%）,光滑念珠菌21株（9.86%）。特康唑对192株白念珠菌的MIC范围：0.024~12.5μg/m L,MIC50：0.39μg/m L,MIC90：1.56μg/m L,GM：0.248μg/m L;特康唑对19株光滑念珠菌的MIC范围：0.024~1.56μg/m L,MIC50：0.049μg/m L,MIC90：1.56μg/m L,GM：0.107μg/m L。结论目前阴道念珠菌病的病原菌仍以白念珠菌为主,并提示特康唑与克霉唑、咪康唑3种药物对213株念珠菌均具有良好的敏感性,特康唑对白念珠菌及光滑念珠菌有较强的抑制作用并未发现有耐药现象。Objective To research the sensitivity of major Candida,isolated from severe vulvovaginal candidiasis to terconazole.Methods Isolates were collected from the patients with vulvovaginal candidiasis,who were from the Gynecology clinic of Shanghai Renji Hospital,Shanghai Sixth People＇s Hospital and Shanghai First Maternity and Infant Hospital.CHROM agar candida medium and API 20 C AUX were used to identify Candida species.The sensitivity of 213 Candida isolates to terconazole,clotrimazole and miconazole were tested by microdilution broth method according to the CLSI M27-A3 microdilution method. Results 192 isolates were identified to C.albicans（ 90.14%）,21 isolates were identified to C.glabrata（ 9.86%）.The sensitivity of C.albicans isolates to terconazole were as follows： MIC range： 0.024-12.5 μg / m L,MIC50： 0.39 μg/m L,MIC90： 1.56 μg/m L,GM： 0.248 μg/m L; The sensitivity of C.glabrata isolates to terconazole were as follows： MIC range： 0.024-1.56 μg / m L,MIC50： 0.049 μg/m L,MIC90： 1.56 μg/m L,GM： 0.107 μg / m L.Conclusions Currently vaginal candidiasis infection is still based on C. albicans. The research reprompted terconazole with clotrimazole,miconazole three kinds of antifungal drugs on the 213 Candida have good sensitivity.Terconazole has strong inhibition to C.albicans and C.glabrata and did not find resistance phenomenon.

关 键 词：CLSIM27-A3 真菌药敏试验 外阴阴道念珠菌病 特康唑 克霉唑 咪康唑 

分 类 号：R969.4[医药卫生—药理学] R379.4[医药卫生—药学]