一种适于丝状真菌基因RNA干扰方法的研究  

An investigation on RNA-interference method applicable for the filamentous fungi

作　　者：张振颖[1] 侯彬彬[2] 刘霞[2] 

机构地区：[1]香港大学深圳医院皮肤科,深圳518053 [2]大连医科大学附属第二医院皮肤科,大连116023

出　　处：《中国真菌学杂志》2015年第3期163-167,共5页Chinese Journal of Mycology

基　　金：国家自然科学基金(81000699);深圳市科创委(JCYJ20140414092023239)

摘　　要：目的获得一种适于丝状真菌基因研究用的、由根癌农杆菌介导的RNA干扰方法。方法采用基因重组及菌株干扰体系筛选的方法。结果获得适于根癌农杆菌转染的重组载体PCB309-pfgrt,并将其成功用于对孢子丝菌双组份信号组氨酸蛋白激酶DRK1基因的干扰中。结论该方法优化了根癌农杆菌的转化体系,解决了丝状真菌RNA干扰载体构建困难及干扰效率低下的难题,在丝状真菌基因功能及遗传转化研究方面具有广泛的应用前景。Objective To obtain a RNA-interference method mediated by Agrobacterium tumefaciens applicable for gene research on filamentous fungi.Methods Gene recombination and interference system selection were used. Results The recombinant vector PCB309-pfgrt was obtained and used in interfering the expression level of two-component DRK1 gene successfully.Conclusions This method optimized the transformation system,solving the problem of high efficient RNA interference vector construction,which has wide application prospect in genetic research in filamentous fungi.

关 键 词：重组载体 RNA干扰 丝状真菌 根癌农杆菌 

分 类 号：R379[医药卫生—病原生物学]