玉米单倍体诱导系JS6-2诱导效果的研究  

Induced rate of maize haploid inducer JS6-2

作　　者：杨巍[1] 任雪娇[1] 崔学宇 慈佳宾[1] 任启彪[2] 杨伟光[1] 吴春胜[1] 

机构地区：[1]吉林农业大学农学院,吉林长春130118 [2]吉林省种子管理总站,吉林长春130062

出　　处：《西北农林科技大学学报（自然科学版）》2015年第7期89-94,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：吉林农业大学科研启动基金项目(201204);国家"863计划"项目(2011AA10A103);农业部948项目(2011-G1-21);国家农转资金项目(2014GB2B100006);吉林省现代农业产业技术体系项目(2013001J-1);国家转基因重大专项(2014ZX800305B);国家科技支撑计划项目(2014BAD01B01)

摘　　要：【目的】探讨玉米单倍体诱导系JS6—2对不同基础材料、不同授粉时期和果穗不同部位的诱导效果，为玉米单倍体育种技术的应用提供理论依据。[方法】以玉米单倍体诱导系JS6—2为诱导材料，Stock6和农大高诱1号为对照，对不同基础材料进行了杂交诱导，同时比较了不同授粉时期、不同果穗部位对单倍体诱导率的影响。【结果]JS6—2对自交系及杂交种均具有诱导产生单倍体的能力，对自交系诱导中，JS6—2单倍体诱导率（8．29％）明显高于农大高诱1号（1．70％）和Stock6（O．66％）；对杂交种诱导中，JS6—2单倍体诱导率（8．22％）同样明显高于其他2个诱导系（1．35％和0．76％）。JS6—2杂交诱导单倍体最佳时期为雌穗吐丝后3～8d；果穗顶部的单倍体诱导率（6．87％）高于底部（5．61％）。【结论】玉米单倍体诱导系JS6—2的诱导效果明显好于农大高诱1号和Stock6；Reid、NoReid材料以及Reid×DOM和NoReid×DOM杂优模式材料的单倍体诱导率高；田间杂交诱导时，建议在雌穗吐丝3～5d整齐剪断花丝进行杂交诱导，单倍体诱导效果好。[Objective] Induction effects of maize haploid inducer JS6-2 with different germplasms, artificial pollination times and sites were studied to improve maize haploid breeding. [Method] Using JS6-2 as inducing line and Stock6 and Cau ho 1 as control,induction rates between inbred lines and hybrids with different artificial pollination times and sites of ear were compared. [Result] JS6-2 had the ability of inducing inbred lines and hybrids. The induction rates of JS6-2 for inbred lines and hybrids were higher than Cau ho I and Stock6. The average induction rates for inbred lines were 8.29%,1.70% and 0.66% ,while those for hybrids were 8.22% ,1.35% and 0. 76%, respectively. [Conclusion] JS6-2 was much more efficient than Cau ho 1 and Stock6 in induction of inbred and hybrid lines. Reid, No Reid, Reid × DOM, and No Reid × DOM had high induction rates. Optimal artificial pollination period for inducer JS6-2 was 3--5 d after silking at top site of ear.

关 键 词：玉米 单倍体诱导系 诱导效果 

分 类 号：S335.4[农业科学—作物遗传育种] S513.035.2[农业科学—农艺学]