气相色谱-质谱法测定大鼠血浆中尼古丁及其药物代谢动力学研究  

Determination of Nicotine and Its Pharmacokinetics in Rat Plasma by GC-MS

作　　者：王嘉乐[1] 李钢[1] 朱云[1] 金永明[1] 吴达[1] 

机构地区：[1]上海烟草集团有限责任公司技术中心,上海200082

出　　处：《理化检验（化学分册）》2015年第7期1003-1006,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

摘　　要：采用气相色谱-质谱法测定大鼠血浆中的尼古丁。血浆样品采用蛋白沉淀法净化,采用DB-Wax毛细管色谱柱分离,质谱中选择电子轰击离子源和选择离子监测模式,以茴香脑为内标进行定量。并采用PKSlover 2.0软件计算药代动力学参数。尼古丁的线性范围为0.10-2.0mg·L^-1,检出限为4.59μg·L^-1。对空白样品进行加标回收试验,回收率在85.0%-90.1%之间,相对标准偏差（n=6）小于3.0%。应用此方法考察了灌胃给药的大鼠血浆样品中尼古丁代谢动力学特点,主要动力学参数AUC0-t,AUC0-∞,Tmax和ρmax分别为8.570mg·L^-1·h^-1,10.49mg·L^-1·h^-1,1.0h,998.9μg·L^-1,结果呈现药物代谢动力学过程。GC-MS was used for the determination of nicotine in rat plasma.The plasma sample was purified by aprotein precipitation method and then separated on a DB-Wax capillary column.EI and SIM were adopted in MS.Anethole was used as the internal standard for quantification.Pharmacokinetic parameters were calculated by using software PKSlover 2.0.The linearity range of nicotine was 0.10-2.0 mg·L^-1 with detection limit of4.59μg·L^-1.The recovery rates measured by standard addition method were in the range of 85.0%-90.1% with RSD less than 3.0%.The method was applied for pharmacokinetic study of nicotine in the rat plasma samples.AUC0-t,AUC0-∞,Tmaxandρmaxwere 8.570mg·L^-1·h^-1,10.49mg·L^-1·h^-1,1.0h,and 998.9μg·L^-1,respectively,displaying apharmacokinetic process.

关 键 词：气相色谱-质谱法 尼古丁 大鼠血浆 药物代谢动力学 

分 类 号：O657.63[理学—分析化学]