Up-regulation of iNOS by hypoxic postconditioning inhibits H9c2 cardiomyocyte apoptosis induced by hypoxia/re-oxygenation  

作　　者：Huanxin Zhao Rong Yang Yujuan Shi Wanfang Yang Qun Zeng Guoyi Zhao Xiaoliang Wang 

机构地区：[1]Department of Physiology, Shanxi University of Traditional Chinese Medicine, Taiyuan 030024, China [2]Center for Cardiovascular Sciences, Albany Medical Center, Albany, NY 12208, USA

出　　处：《Acta Biochimica et Biophysica Sinica》2015年第7期516-521,共6页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （Nos. 81100150 and 81170144）, and the Natural Science Foundation of Shanxi Province （No. 2012011040-1 ）.

摘　　要：Apoptosis is a crucial mode of cell death induced by ischemia and reperfusion, and ischemic postconditioning （PostC） has been reported to inhibit cell apoptosis. Inducible nitric oxide synthase （iNOS） has been confirmed to play an important role in triggering and mediating the late cardio-protection against ischemia/hypoxia. In this study, we found that hypoxic PostC remarkably up-regulated the expression of iNOS and decreased cardiomyocyte apoptosis. Pre-treatment with 1400w （a highly selective inhibitor of iNOS） or iNOS siRNA weakened the anti-apoptotic effect of hypoxic PostC. These findings suggested that iNOS may be one of the key molecular mechanisms responsible for the inhibition of apoptosis by hypoxic PostC.Apoptosis is a crucial mode of cell death induced by ischemia and reperfusion, and ischemic postconditioning （PostC） has been reported to inhibit cell apoptosis. Inducible nitric oxide synthase （iNOS） has been confirmed to play an important role in triggering and mediating the late cardio-protection against ischemia/hypoxia. In this study, we found that hypoxic PostC remarkably up-regulated the expression of iNOS and decreased cardiomyocyte apoptosis. Pre-treatment with 1400w （a highly selective inhibitor of iNOS） or iNOS siRNA weakened the anti-apoptotic effect of hypoxic PostC. These findings suggested that iNOS may be one of the key molecular mechanisms responsible for the inhibition of apoptosis by hypoxic PostC.

关 键 词：inducible nitric oxide synthase hypoxic postconditioning apoptosis CARDIOMYOCYTE 

分 类 号：Q343.1[生物学—遗传学] S852.3[农业科学—基础兽医学]