导浊排毒颗粒中蒽矾类五种成分的含量测定  

Determination of Anthraquinones Compounds in Daozhuo Paidu GranuL es and Determination of the Content of Aloe Emodin

作　　者：张仁霞 蔡琼谅 岑志芳[2] 黄英晓 

机构地区：[1]广东佛山市南海区第九人民医院,佛山528000 [2]广东佛山科学技术学院,佛山528000

出　　处：《海峡药学》2015年第6期58-60,共3页Strait Pharmaceutical Journal

摘　　要：目的建立导浊排毒颗粒中大黄素甲醚、芦荟大黄素、大黄酚、大黄酸、及大黄素含量测定的方法;方法色谱柱:Agilent ZORBAX SBC18(4.6×150mm,5μm),甲醇-0.1%磷酸为流动相以梯度洗脱;流速:1m L·min-1,检测波长为254nm,柱温25℃,进样量20μL;结果芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚和制剂中其他成分可完全分离,芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的平均回收率分别为:96.6%、96.5%、96.8%、97.1%、96.8%;平均RSD%分别为:1.41%、1.04%、0.89%、1.06%、1.78%(n=6);结论该方法专属性强,重现性好,可用于导浊排毒颗粒中蒽醌类成分的含量测定。OBJECTIVE To establish a method to determine the content of emodin,rhein,chrysophanol,emodin,aloe emodin in Daozhou Paidu granules. METHODS Chromatographic column was Agilent ZORBAX SB-C18（ 4. 6 × 150 mm,5μm）. Methanol-0. 1% phosphate as mobile phase in gradient elution; Velocity was 1m L·min- 1with the detection wavelength of 254 nm and the column temperature of 25℃; The injection volume was20μL. RESULTS Emodin,Rhein,Chrysophanol,Physcion,Aloe Emodin and other ingredients were separated completely. The average recovery rate were 90. 51% 、90. 80% 、91. 14% 、91. 99% 、91. 55%. The average RSD were2. 69% 、5. 90% 、3. 23% 、2. 76% 、2. 13%（ n = 6） respectively. CONCLUSION This method is specific,reproducible,and can be used for determination of the content of the anthraquinones compounds in turbid toxin.

关 键 词：导浊排毒颗粒 大黄酸 大黄素 大黄酚 高效液相色谱 

分 类 号：R927.2[医药卫生—药学]