金银花商品规格等级标准研究  

Study on Commercial Specification of Lonicerae Japonicae Flos

作　　者：周洁[1,2] 邹琳[3] 刘伟[1] 边丽华[3] 王晓[1] 张永清[3] Dan Staerk 

机构地区：[1]山东省分析测试中心/山东省科学院,山东济南250014 [2]道地药材国家重点实验室/中国中医科学院中药资源中心,北京100700 [3]山东中医药大学,山东济南250355

出　　处：《中药材》2015年第4期701-705,共5页Journal of Chinese Medicinal Materials

基　　金：国家重点实验室建设项目计划(2060302);国家自然科学基金(81130070);山东省自主创新专项(2013CSC20401);山东省海外高层次人才资助专项资金

摘　　要：目的:为金银花商品规格等级标准的修订提供基础数据。方法:收集不同商品等级金银花样品39份,用游标卡尺和电子天平对每0.5 g花蕾数、每0.5 g开花率、杂质含量、褐变、霉变、花蕾长度、上部直径、中部直径和下部直径等外观性状进行测量;采用HPLC法对新绿原酸、绿原酸、隐绿原酸、芦丁、木犀草苷、3,5-二咖啡酰奎宁酸和4,5-二咖啡酰奎宁酸等活性成分含量进行检测;利用SPSS统计软件进行相关性分析、主成分分析和聚类分析,并对外观性状指标和内在活性成分含量指标间进行相关性分析。结果:每0.5 g花蕾数、每0.5 g开花率、杂质含量、褐变为主成分指标。花蕾长度与木犀草苷含量具有极显著相关性,褐变、上部直径与绿原酸含量具有显著相关性。结论:以筛选的指标将金银花商品划分为4等。Objective： To provide the basis data for the institution of commercial specification standard of Lonicerae Japonicae Flos. Methods： 39 samples of Lonicerae Japonicae Flos commercial of different grades in market were collected,and vernier caliper and electronic balance were used to measure the numbers of flower bud and blooming rate per 0. 5 g,contamination content,browning degree,milden and rot,length,upside diameter,middle diameter and bottom diameter of Lonicerae Japonicae Flos. The content of neochlorogenic acid,chlorogenic acid,cryptochlorogenic acid,rutin,galuteolin,3,5-dicaffeoylquinic acid and 4,5-dicaffeoylquinic acid were detected by HPLC. Correlation analysis,principal component analysis and cluster analysis were used by SPSS to analyze all index data,and the correlation of appearance characteristics and intrinsic active constituents was discussed. Results： The numbers of flower bud and blooming rate per 0. 5 g,contamination content and browning degree were principal component indexes. The length of flower bud showed a significant correlation with galuteolin content,and the browning degree and upside diameter showed a significant correlation with chlorogenic acid content. Conclusion： Lonicerae Japonicae Flos commercial should be divided into four specification grades by sieved indexes.

关 键 词：金银花 商品规格等级 外观性状 活性成分 

分 类 号：R282.71[医药卫生—中药学]