水线草UPLC指纹图谱的建立及应用  

UPLC Fingerprint of Oldenlandia corymbosa

作　　者：李洪权[1] 曹艺[1] 柏玉冰 夏伯候[1] 林丽美[1] 廖端芳[1] 

机构地区：[1]湖南中医药大学/湖南省中药不良成分快速检测及脱除工程技术研究中心/中药有毒物质防控技术湖南省工程实验室,湖南长沙410208

出　　处：《中药材》2015年第4期735-738,共4页Journal of Chinese Medicinal Materials

基　　金：中国药典2015版一部标准研究项目(Z18);湖湘青年科技创新创业平台人才项目(2013);湖南省十二五重点学科药学学科资助

摘　　要：目的:建立不同产地水线草的超高效液相色谱法(UPLC)指纹图谱,并对其混淆品白花蛇舌草进行鉴别应用。方法:采用UPLC法,色谱柱为ACQUITY UPLC BEH C18(50 mm×2.1 mm,1.7μm),以甲醇-1%冰醋酸梯度洗脱,柱温30℃,流速0.2 m L/min,检测波长254 nm。指纹图谱采用相似度分析和主成分分析。结果:建立了水线草药材的专属性指纹图谱,确定了15个共有峰。各产地水线草药材指纹图谱相似度均超过0.85。结论:该指纹图谱重复性好,简便可靠,可用于水线草药材的专属性鉴别,有效区别水线草及其混淆品白花蛇舌草。Objective： To establish the UPLC fingerprint of Oldenlandia corymbosa from different regions and to distinguish it from Oldenlandia diffusa. Methods： UPLC procedure was performed on an ACQUITY UPLC BEH C18（ 50 mm × 2. 1 mm,1. 7 μm） column and eluted with a mobile phase consisted of methanol-1% acetic acid at a flow rate of 0. 2 m L / min. The column temperature was 30 ℃. The detection wavelength was 254 nm. A matrix was constructed for similarity evaluation,cluster analysis and principle component analysis. Results： The collected samples had a good similarity. A specificity fingerprint chromatogram was produced and 15 common peaks were designated. Samples were divided into four groups. Conclusion： It is a reliable and available method for specific identification of Oldenlandia corymbosa and for distinguishing Oldenlandia corymbosa and Oldenlandia diffusa.

关 键 词：水线草 白花蛇舌草 UPLC指纹图谱 相似度 聚类分析 主成分分析 

分 类 号：R282.5[医药卫生—中药学]