血管内皮生长因子长效缓释微球不同配比对体外释放行为改善的研究  

Effect of Different Mixture Ratios of VEGF-loaded PLGA Microspheres on Release Behavior in Vitro

作　　者：刘剑锋[1] 陈韵岱[2] 

机构地区：[1]解放军总医院南楼心内科,北京100853 [2]解放军总医院心内科,北京100853

出　　处：《解放军药学学报》2015年第3期224-227,243,共5页Pharmaceutical Journal of Chinese People's Liberation Army

摘　　要：目的用生物可降解聚乳酸羟基乙酸共聚物(PLGA)制备载药微球包埋血管内皮生长因子(VEGF),并探索不同配比对释放行为的影响。方法采用不同分子量的PLGA制备不同粒径的载药微球,并经载药微球的合理配比改善其体外释放行为,达到优化工艺、降低成本的目的。结果载药微球粒径约为20μm、分子量10 kU:24 kU的配比为1:2组,粒径为20μm、分子量为24 kU和分子量为10 kU、粒径为6μm的载药微球配比为2:1组的体外释放突释较低,且在14 d内呈线性的零级释放趋势,体外释放行为得到改善。结论 VEGF长效缓释PLGA微球经优化配比后的持续释放能力较传统VEGF微球明显提高。Objective To explore the optimal mixture ratio of vascular endothelial growth factor（VEGF）-loaded poly（lactic-co-glycolic） acid（PLGA） microspheres prepared by different formulations and to achieve constant release.Methods PLGA microspheres of different molecular weights and sizes were prepared,which were mixed at different mass ratios to improve in vitro release behavior.This method not only reduced the workload of optimization and cost,but improved the in vitro release behavior.Results The optimal ratios：（1） the size of microspheres was 20 μm,and mass ratio of 10 kU：24 kU was 1：2;（2） the mass ratio between microspheres of molecular weight 24 kU and size 20 μm and those of molecular weight 10 kU and size 6 μm was 2：1.The in vitro release behavior at these two ratios presented low burst release and zero-order release within 14 days,which was worthy of further study in vivo.Conclusion The long-term release behavior of VEGF-loaded PLGA microspheres mixed by two formulations has been greatly improved compared with the traditional one.

关 键 词：血管内皮生长因子 聚乳酸羟基乙酸共聚物 微球 长效缓释 配比 

分 类 号：R94[医药卫生—药剂学]