小鼠和大鼠的胚胎培养基及若干相关问题  

Culturing of Mouse and Rat Preimplantation Embryos in Various Chemically Defined Media

作　　者：高珊 陈炜 于磊 李静[1] 孙彩显 高杰 刘牧[1,2] 

机构地区：[1]中国医学科学院中国医学实验动物研究所,北京100021 [2]中国疾病预防控制中心,北京100050

出　　处：《中国生物工程杂志》2015年第7期83-93,共11页China Biotechnology

基　　金：国家科技支撑计划资助项目(2014BA202B01)

摘　　要：哺乳动物早期胚胎体外培养所用各种化学成份明确的培养基是研究生命科学中一项重要技术。它已被常规用于研究胚胎早期发育,多种动物及人类辅助生殖技术,转基因动物,动物克隆等领域。介绍了用于移植前胚胎培养基的研究和发展历史,当前所用化学成份明确胚胎培养基的主要组成,特别是针对小鼠和大鼠移植前胚胎所用各种培养基及其成份,讨论了这类培养基发展前景和研究方向。The mammalian oocytes were in vitro fertilization （IVF）, the preimplantation embryos （PE） were in vitro cultured （IVC） that was one of important technical in the modem bio-science. The normally to be used medium, one had been called chemically defined medium （CDM）. It had been used in a wildly field, frequently. It included in the animal and human＇ s artificial assisted reproductive technical （ART） , to research the embryo of earlier developing stage, the transgenic animal, and the animals clone, etc. The review was included in the development history and its constituents of the CDM. There was also included some modified embryo media, and focused on the especial media, which was to be used for culturing the mouse or rat embryo. Meanwhile, the article had introduced the prospects and research direction of CDM, briefly.

关 键 词：移植前胚胎 体外成熟 体外受精 体外培养 培养基 化学成份明确的培养基 

分 类 号：Q78[生物学—分子生物学]