应用肠杆菌科基因间重复序列聚合酶链反应对鲍曼不动杆菌进行同源性分析  

Study on the homology of Acinetobacter baumannii by enterobacterial repetitive intergenic consensuspolymerase chain reaction

作　　者：闻海丰[1] 秦瑾[2] 于文静[3] 冯彦蕊[1] 冯忠军[1] 

机构地区：[1]河北医科大学第三医院检验科,河北石家庄050051 [2]河北医科大学第三医院感控科,河北石家庄050051 [3]河北省儿童医院检验科,河北石家庄050031

出　　处：《分子诊断与治疗杂志》2015年第4期247-252,共6页Journal of Molecular Diagnostics and Therapy

基　　金：河北省卫生厅重点科技研究计划(20110099)

摘　　要：目的了解临床不同来源的鲍曼不动杆菌是否存在同源性,判断其是否存在耐药菌株的克隆播散,追踪其在医院交叉感染的可能途径,以便有效防控鲍曼不动杆菌在医院中传播。方法收集2012年10月至2013年6月临床分离的73株多重耐药鲍曼不动杆菌,用肠杆菌科基因间重复序列-聚合酶链反应(enterobacterial repetitive intergenic consensus-polymerase chain reaction,ERIC-PCR)对菌株进行同源性分析。结果 73株多重耐药鲍曼不动杆菌经ERIC-PCR分型分为8个基因型,分别用A、B、C、D、E、F、G、H表示,其中A型31株,B型15株,C型12株,D型8株,E型3株,F型2株,G和H型各为1株。A型为主要的流行株,分布在多个不同的科室。重症监护室(intensive care unit,ICU)分离的菌株存在6个基因型;骨科系统病区存在4个基因型;急诊科有5个基因型;内科发现有6个基因型;外科发现3个基因型。ICU病房环境物体表面共分离出4株鲍曼不动杆菌,其中9号和10号菌株来自于不同患者的床旁,12号和13号来自于护理站电脑键盘。结论临床分离的多重耐药鲍曼不动杆菌存在多个基因型。多重耐药鲍曼不动杆菌存在时间和空间的聚集性。Objective To investigate the homology of clinical isolated Acinetobacter baumannii and the epidemiology of the possible transmission routes. Methods 73 isolates of non-repetitive multidrug resistant Acinetobacter baumannii were collected from October 2012 to June 2013. Epidemiological typing was performed by enterobacterial repetitive intergenic consensus-polymerase chain reaction（ERIC-PCR）. Results The 73 isolates of Acinetobacter baumannii were classified into 8 distinct patterns by ERIC-PCR, including31 of genotype A, 15 of genotype B, 12 of genotype C, 8 of genotype D, 3 of genotype E, 2 of genotype F, 1of genotype G, and 1 of genotype H. Genotype A was the dominating clone and distributed in different wards.There were 6 genotypes in ICU unit, 4 genotypes in the department of orthopedics, 5 genotypes in emergency department, 6 genotypes in internal medicine and 3 genotypes in surgical department, respectively. There were 4 isolates of Acinetobacter baumannii on the surface of ICU unit. No. 9 and No. 10 isolates were isolated from the bedsides of different patients, and No. 12 and No. 13 were isolated from the computer keyboard in nurses＇ station. Conclusion The isolates of Acinetobacter baumannnii in our hospital have multiple genotypes. There were temporal and spatial aggregations in the infection of Acinetobacter baumannnii in our hospital.

关 键 词：鲍曼不动杆菌 多重耐药性 肠杆菌科基因间重复序列-聚合酶链反应 同源性 

分 类 号：R446.5[医药卫生—诊断学]