ATP荧光检测法检测医院电梯按钮表面细菌总数  

Detection of Surface Microbial Count on Elevator Buttons of Hospital Using ATP Bioluminescence Method

作　　者：刘天钊[1] 李晓青[2] 刘俊江[2] 张艳玲[2] 梁岩[2] 

机构地区：[1]北京大学深圳医院,深圳518036 [2]中国科学院深圳先进技术研究院食品安全及环境技术研究室,深圳518055

出　　处：《集成技术》2015年第4期82-86,共5页Journal of Integration Technology

摘　　要：使用ATP荧光快速检测法测定了北京大学深圳医院16部电梯按钮表面的细菌总数。按照GB15982-2012评价标准评价,消毒前合格率为72.92%,消毒后合格率为93.75%。ATP发光强度对数值与表面细菌总数对数值线性关系为y=1.12048x-0.66611,相关系数R2=0.95797。分析主要影响因素为人员类型及人流量。ATP荧光检测法能够快速、准确对医院电梯按钮表面细菌总数进行测定,可以有效反映医院公共卫生状况,防止交叉感染。Microbial count on surface of buttons in 16 elevators in Peking University Shenzhen Hospital was detected using ATP bioluminescence method. The quantifi cation signifi cantly correlated with the pour plate method（y=1.12048x-0.66611, R2=0.95797）. According to the GB15982-2012 standard, about 72.92% unsterilized samples and 95.83% sterilization samples were acceptable. Factors affecting general hygiene condition in elevators were mainly people types and fl ux. In general, ATP bioluminescence method was demonstrated to be a reliable fast testing method for quantifying microbial contamination on surface of elevator buttons, which therefore could serve as a good indicator of public hygiene surveillance and management in hospitals. This is important for preventing cross-infection.

关 键 词：电梯 表面细菌总数 ATP荧光 医院清洁 

分 类 号：R197.32[医药卫生—卫生事业管理]