贵州东北部黑叶猴种群mtDNA D-loop序列多态性  

Sequence polymorphism of mt DNA D- loop of Franois' langurs( Trachypithecus francoisi) in northeastern Guizhou,China

作　　者：胡娟[1] 陈媛[1] 邓怀庆[1] 谷晓明[1] 李志刚[1] 周江[1] 

机构地区：[1]贵州师范大学生命科学学院,贵阳550001

出　　处：《兽类学报》2015年第3期336-341,共6页Acta Theriologica Sinica

基　　金：贵州省科技基金(黔科合LH字[2014]7057);贵州师范大学博士科研启动基金(11904-0514017)

摘　　要：贵州东北部作为黑叶猴在中国的主要分布区之一,黑叶猴的数量在近30年急剧下降,栖息地破碎化严重,保护形势不容乐观。深入了解黑叶猴在贵州东北部地区地理种群的分子变异及遗传水平现状,可以为黑叶猴保护提供重要的依据。本文以黑叶猴的粪便、毛发为实验材料,利用线粒体DNA分子标记,对采自贵州东北部地区黑叶猴野生种群79个粪便样品和黔灵公园笼养种群22个粪便样品、5份毛发样品的线粒体控制区(mt DNA D-loop)的部分序列进行测定。贵州东北部黑叶猴样品共获得88条395 bp的序列,共有30个多态位点,其碱基突变形式为转换和颠换,无插入和缺失,共6个单倍型。构建的系统发育树表明,贵州黑叶猴是一个独立的单元群,研究结果有助于了解贵州东北部地区野生黑叶猴的遗传多样性,分析其种群适应能力与进化潜力,为提出科学合理的保护管理对策提供参考依据。Northeastern Guizhou is one of the main distribution areas of the Francois＇ langur （Trachypithecus francoisi） in China, however, the habitat has been seriously damaged and population size of the Francois＇ langur has declined very quickly, showing a distribution peculiarity of fragmentation in the northeastern Guizhou. Therefore, understanding the mor- phological and molecular variation of different geographic groups of the Francois＇ langur in these areas is of vital importance in designing conservation strategies. We used 79 faecal samples to investigate their mitoehondrial DNA D-loop, all of which were collected from the wild Francois＇ langur population in northeastern Guizhou. An additional 22 feces samples and 5 hair samples which were collected from the captive population in the Qianling Park of Guiyang were also tested. Finally, we obtained 88 sequences of this region （ D-loop）, containing 395 base pair fragment nucleotides. We identified 30 variable nucleotide sites of transitions and transversions, whereas no insertions/deletions was detected. 6 haplotypes were defined and used to construct the population phylogenetic tree, which revealed the Francois＇ langur as an independent unit. This is helpful for studying the genetic diversity and conservation status of the wild Trachypithecus francoisi in northeast Guizhou, and important for designing conservation strategies based on the evaluation of their adaptation ability and evolutionary poten- tial.

关 键 词：黑叶猴 线粒体控制区 单倍型 遗传多样性 

分 类 号：Q346.3[生物学—遗传学]