新风胶囊对佐剂性关节炎模型大鼠的抗炎作用  

Anti-inflammatory Effect of Xinfeng Capsule on Adjuvant Arthritis Model Rats

作　　者：张静[1] 孟楣[1,2] 王晓玉[2] 葛平[1] 江莹[1] 张贺[1] 王芳[1] 

机构地区：[1]安徽中医药大学研究生部,合肥230038 [2]安徽中医药大学第一附属医院/国家中医药管理局中药制剂三级实验室,合肥230031

出　　处：《中国药房》2015年第22期3083-3086,共4页China Pharmacy

摘　　要：目的:研究新风胶囊对佐剂性关节炎(AA)模型大鼠的抗炎作用。方法:取70只SD大鼠随机均分为正常对照组、模型对照(等容生理盐水)组、阳性对照中药[祛风止痛胶囊0.4 g(生药)/kg]组、阳性对照西药(来氟米特片2.1 mg/kg)组和新风胶囊低、中、高剂量[0.8、1.6、3.2 g(生药)/kg]组,依次记为A、B、C、D、E、F、G组,除A组外其余各组大鼠皮内注射弗氏完全佐剂复制AA模型,致炎第12天开始连续ig给药28 d。检测并计算各组大鼠给药前、后足趾肿胀度和关节炎指数,于末次给药24 h后检测各组大鼠血清及关节滑膜组织中白细胞介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)含量并观察踝关节组织病理学形态变化。结果:与A组比较,B组大鼠足趾肿胀度和关节炎指数升高,血清及关节滑膜组织中IL-1β、TNF-α含量增加,差异有统计学意义(P<0.01)。与给药前比较,C、D、E、F、G组大鼠足跖肿胀度和关节炎指数降低,差异有统计学意义(P<0.05)。与B组比较,C、D、E、F、G组大鼠足趾肿胀度和关节炎指数降低,血清及关节滑膜组织中IL-1β、TNF-α含量降低,差异有统计学意义(P<0.01或P<0.05)。B组大鼠踝关节组织出现滑膜增生、炎性细胞浸润;C、D、F、G组大鼠滑膜增生和炎症情况较B组均减轻。结论:新风胶囊对AA模型大鼠具有抗炎作用,其机制可能与促进滑膜细胞凋亡、抑制滑膜增生有关。OBJECTIVE：To study the anti-inflammatory effect of Xinfeng capsule on adjuvant arthritis（AA）in rats.METHODS：70 SD rats were randomized into a normal control group（isovolumetric normal saline）,a model control group（isovolumetric normal saline）,a positive control TCM group [Qufeng zhitong capsule 0.4 g（crude drug）/kg],a positive control chemical medicine group（Leflunomide tablet 2.1 mg/kg）and groups of low,middle and high doses of Xinfeng capsule,which were respectively marked as groups A,B,C,D,E,F and G.All groups of rats except for Group A were given freund＇s complete adjuvant intracutaneous injection to establishe AA models,and from the 12 th day after the inflammation was induced,were given the corresponding drug,ig,for 28 consecutive days.The degree of toe swelling and arthritis index were determined and calculated for all groups of rats before and after the administration.The contents of interleukin 1β（IL-1β）and tumor necrosis factor α（TNF-α）in serum and synovial membrane of all groups of rats were determined 24 h after the last administration,and pathomorphological changes of their ankles were observed by light microscope.RESULTS：Compared to group A,group B demonstrated higher degree of toe swelling,arthritis index and contents of IL-1β and TNF-α in serum and synovial membrane,with statistical significance（P〈0.01）.After the administration,the degree of toe swelling and arthritis index of groups C,D,E,F and G reduced,with statistical significance（P〈0.05）.Compared to group B,groups C,D,E,F and G showed lower degree of toe swelling,arthritis index and contents of IL-1β and TNF-α in serum and synovial membrane,with statistical significance（P〈0.01 or P〈0.05）.Synovial membrane hyperplasia and inflammatory cell infiltration were noted in the ankle tissue of the rats in group B,and such symptoms were not so serious in groups C,D,F and G.CONCLUSIONS：Xinfeng capsule has anti-inflammatory effect for AA rats by a mechanism which may be related to accelera

关 键 词：新风胶囊 佐剂性关节炎 大鼠 抗炎 踝关节组织病理形态 

分 类 号：R285[医药卫生—中药学]