骨愈方中总黄酮的提取工艺研究  

Extraction Technology of Total Flavonoids for the Bone Healing Formulation

作　　者：高瑛 赵淑慧 陆洋[2] 温然[2] 

机构地区：[1]北京中医医院顺义医院,北京101300 [2]北京中医药大学中药学院,北京100102

出　　处：《中国药房》2015年第22期3125-3127,共3页China Pharmacy

基　　金：北京市中医药科技项目(No.JJ2011-36)

摘　　要：目的:建立骨愈方中总黄酮的最优提取工艺以用于制剂研究。方法:以总黄酮含量为指标,通过L9(34)正交试验法考察溶剂乙醇体积分数、溶剂用量、提取次数、提取时间对骨愈方中总黄酮提取的影响,确定各因素的最优水平并进行验证试验。结果:总黄酮的最优提取工艺为10倍药材量的70%乙醇溶剂回流提取3次,每次1 h;验证试验得总黄酮的平均含量为62.03 mg/ml(RSD=0.84%,n=3),即从100 g处方药材可提取6.20 g总黄酮成分。结论:优选工艺稳定可行,可用于骨愈方中总黄酮的提取,可为骨愈搽剂的制备提供试验依据。OBJECTIVE：To optimize the extraction technology of total flavonoids for Bone healing formulation in order to use it for preparation research.METHODS：With the content of total flavonoids as the index,L9（34）orthogonal test was employed to investigate the effects of volume fraction of the solvent ethanol,the amount of solvent,extraction times and extraction time on the extraction of total flavonoids for Bone healing formulation to determine the optimal levels of the factors,and verification tests were conducted.RESULTS：The optimal extraction technology of total flavonoids was 1 h reflux extraction for 3 times,with 70% ethanol 10 times as much as the amount of medicinal materials.Verification tests showed the average content of total flavonoids was62.03 mg/ml（RSD=0.84%,n=3）,that is to say,6.20 g total flavonoids might be extracted from 100 g medicinal materials for the formulation.CONCLUSIONS：The optimal technology is stable and feasible and can be used for the extraction of total flavonoids for Bone healing formulation and provide a experimental basis for the preparation of Bone healing liniment.

关 键 词：骨愈方 搽剂 提取工艺 总黄酮 正交试验 

分 类 号：R284[医药卫生—中药学]