正交试验优选蛇六谷减毒炮制工艺  

Optimization of Toxicity Attenuation Processing Technology of Amorphophallus sinensis Belval.by Orthogonal Test

作　　者：梅新路[1] 徐斌[1] 徐菲拉[1] 仝淑花[1] 何三民[1] 

机构地区：[1]金华市中心医院,浙江金华321000

出　　处：《中国药房》2015年第22期3137-3139,共3页China Pharmacy

摘　　要：目的:优选蛇六谷减毒炮制工艺。方法:以蛇六谷口尝刺激性、兔眼刺激性综合评分为指标,在单因素试验的基础上设计正交试验,考察饱和氢氧化钙溶液用量、加热时间、加热温度对炮制效果的影响,并进行验证试验及炮制前后样品刺激性比较。结果:饱和氢氧化钙溶液用量、加热温度对炮制效果有显著影响;最优炮制工艺为100℃下加入30倍药材量的饱和氢氧化钙溶液加热30 min;验证试验综合评分分别为8.05、8.44、8.37(RSD=2.5%,n=3);炮制前、后综合评分平均值分别为0.12、8.54(n=10)。结论:与炮制前比较,药材经优选工艺炮制后刺激性降低;优选工艺稳定可靠。OBJECTIVE：To optimize the toxicity attenuation processing technology of Amorphophallus sinensis Belval..METHODS：With the overall score of taste stimulation and rabbit eye irritation of A.sinensis Belval.as the index,and based on the single factor method,orthogonal test was designed to investigate the the influences of the amount of saturated calcium hydroxide solution,heating time and heating temperature on the processing effect,and verification tests as well as irratation comparison before and after processing were conducted.RESULTS：The amount of saturated calcium hydroxide solution and heating temperature had significant influence on the processing effect.The optimal processing technology was to add saturated calcium hydroxide solution 30 times as much as the amount of medicinal materials at 100 ℃ and heat it for 30 min.The verification tests showed overall scores of8.05,8.44 and 8.37（RSD=2.5%,n=3）.The average overall scores before and after processing were 0.12 and 8.54（n=10）respectively.CONCLUSIONS：The medicinal materials processed by the optimal technology have lower stimulation and irritation.The optimal technology is stable and reliable.

关 键 词：蛇六谷 正交试验法 减毒炮制 工艺优化 综合评分 

分 类 号：R284[医药卫生—中药学]