盐碱胁迫下玄参幼苗的抗性生理响应研究  

Study on Resistance Physiological Responses of Seedling of Scrophularia ningpoensis to Saline-alkali Stress

作　　者：付英杰[1] 曲晓欢[1] 李艳芝[1] 王建安[1] 

机构地区：[1]济宁医学院药学院,山东日照276826

出　　处：《中国药房》2015年第22期3165-3168,共4页China Pharmacy

基　　金：山东省医药卫生科技发展计划项目(No.2013WS0333);济宁医学院重点课题(No.JYZ013KJ007)

摘　　要：目的:研究不同浓度盐碱胁迫条件下玄参幼苗的抗性生理响应。方法:玄参幼苗置于光照培养箱中,控制温度(20±2)℃、光暗周期13 h/11 h、光照强度2 000 lx,以50、100、150、200、250 mmol/L碱(Na HCO3),50、100、150、200、250 mmol/L盐(Na Cl)浇灌,每4 d 1次,连续16 d。检测幼苗生长指标(株高、鲜质量、干质量)、叶绿素(Chl)含量、抗氧化指标[超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、过氧化氢(H2O2)、丙二醛(MDA)、膜透性(RMP)]水平、渗透调节生理指标[可溶性糖(SS)、可溶性蛋白(SP)、脯氨酸(Pro)]水平。试验设空白对照(蒸馏水)。结果:盐浓度大于50 mmol/L、碱浓度大于150 mmol/L时,幼苗株高降低,鲜质量减少,Chl含量减少;盐浓度大于100 mmol/L、碱浓度大于150 mmol/L时,幼苗干质量减少。盐浓度为50mmol/L、碱浓度为150 mmol/L时,SOD、POD活性最强;盐浓度为150 mmol/L、碱浓度为50 mmol/L时,CAT活性最强。随盐碱浓度升高,H2O2、MDA含量逐渐增加,SP含量逐渐减少;随盐碱浓度增加,RMP水平升高,即膜稳定性逐渐降低;当盐浓度大于100mmol/L、碱浓度大于150 mmol/L时,SS量停止积累;当盐碱浓度大于100 mmol/L时,Pro含量停止累积,但碱浓度下Pro含量明显高于盐浓度下。结论:盐碱胁迫使玄参植物组织产生活性氧,细胞质膜系统受到破坏。Chl、酶促抗氧化剂SOD、CAT、POD,渗透调节剂Pro、SS在对抗盐碱时均起到一定缓冲作用。玄参幼苗能耐受的最高盐浓度为50 mmol/L,最高碱浓度为150 mmol/L。OBJECTIVE：To study the resistance physiological responses of seedling of Scrophularia ningpoensis to the stress caused by saline and alkali at different concentrations.METHODS：The seedling of S.ningpoensis was placed in the illumination incubator,in which the temperature was maintained at（20±2）℃,light-dark cycle at 13 h/11 h and illumination intensity at 2 000 lx.The alkali（Na HCO3）of 50,100,150,200 and 250 mmol/L and the saline（Na Cl）of 50,100,150,200 and 250 mmol/L were poured once every 4 days for 16 consecutive days.The growth indexes（plant height,fresh weight,dry weight）,chlorophyll（Chl） content,antioxidative indexes [superoxide dismutase（SOD）,catalase（CAT）,peroxidase（POD）,hydrogen peroxide（H2O2）,malonaldehyde（MDA）,membrane penetration（RMP）],and osmotic regulation physiological indexes [soluble sugar（SS）,soluble protein（SP）and proline（Pro）] of the seedling were determined.There set a blank control group（distilled water）.RESULTS：For saline concentration higher than 50 mmol/L and alkali concentration higher than 150 mmol/L,the seedling height,fresh weight and Chl content were lower;for saline concentration higher than 100 mmol/L and alkali concentration higher than 150mmol/L,the dry weight of the seedling was lower.For saline concentration of 50 mmol/L and alkali concentration of 150 mmol/L,the activities of SOD and POD were the strongest;for saline concentration of 150 mmol/L and alkali concentration of 50 mmol/L,the activity of CAT was the strongest.The contents of H2O2 and MDA increased and the content of SP decreased with the increase in saline-alkali concentration.The level of RMP increased therewith,that is to say,membrane stability decreased.For saline concentration higher than 100 mmol/L and alkali concentration higher than 150 mmol/L,the accumulation of SS terminated;for saline and alkali concentrations higher than 100 mmol/L,the accumulation of Pro terminated,however,the content of Pro was markedly higher in the above-said alkali co

关 键 词：玄参 盐碱胁迫 生长指标 抗氧化生理指标 渗透调节指标 

分 类 号：R931.2[医药卫生—生药学] Q945.78[医药卫生—药学]