消癌解毒方对移植性肝癌大鼠免疫功能影响研究  

Study of Xiao'ai Jiedu Prescription Effects on Immunity of Rats with Transplanted Hepatoma

作　　者：李文婷[1] 周红光[1] 刘颖 李沐涵[1] 李黎[1] 吴勉华[1] 

机构地区：[1]南京中医药大学,江苏省中医药防治肿瘤协同创新中心,江苏南京210023 [2]南京江宁中医院,江苏南京211100

出　　处：《中华中医药学刊》2015年第8期1880-1883,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81273717);江苏省普通高校研究生科研创新计划项目(CXLX12_0572)

摘　　要：目的:观察消癌解毒方对Walker256大鼠T淋巴细胞亚群和自然杀伤细胞(natural kill,NK)的影响。方法:建立Walker256大鼠模型,50只雄性SD大鼠分为正常组,模型组,中药低剂量组,中药中剂量组,中药高剂量组,每组10只。造模后中药各剂量组进行中药干预,正常组,模型组未进行药物干预。在造模后第10天用流式细胞仪分别检测各组大鼠胸腺,骨髓,外周血T淋巴细胞亚群及NK细胞活性。结果:消癌解毒方干预后,可见低剂量、中剂量组大鼠胸腺指数明显上升,中剂量组大鼠脾指数明显上升,与模型组比较,差异均有显著性(P<0.05);中剂量、高剂量组大鼠骨髓、胸腺细胞与模型组相比,G0/G1期比例显著降低(P<0.05),S期比例明显升高(P<0.05);中剂量组CD4+/CD8+比值明显上升,与模型组相比,差异有显著性(P<0.05);各剂量组NK活性明显增高(P<0.01)。结论:消癌解毒方对Walker256大鼠胸腺指数、脾指数及骨髓、胸腺细胞周期有一定的调节作用,对淋巴细胞亚群CD4+、CD8+、CD4+/CD8+比值及NK细胞活性有一定的调节作用。消癌解毒方具有免疫保护作用可能是其抗癌作用机制之一。Objective：To study the effect of Xiao＇ai Jiedu Prescription （XJP）on T lymphocyte subsets and natural killer cells in Walker256 rat model. Methods ： Establish Walker256 rat model. 50 male SD rats were divided into normal group, model group, low - dose group, middle - dose group and high - dose group. Treatment group was fed a dose - related XJP. Each group＇s weight ,thymus index and spleen index were observed. Groups＇bone marrow and thymus cells and T cell subsets were detected by flow cytometry. NK kit detected NK cells. Results ： It showed that XJP low - dose, middle - dose groups＇thymus indexes were significantly increased. The middle -dose group＇s spleen index was significantly increased in rats. Compared with the model group, the differences were statistically significant （ P 〈 0.05 ）. Compared to the model group ,the high- dose group rat bone marrow and thymus ceils GO/GI phase were significantly decreased （P 〈 0.05）. Sphase was significantly increased （ P 〈 0.05 ）. Compared to the model group, the middle - dose group of CD4＋/CD8＋ was significantly increased （ P 〈 0.05 ）. NK activity of each dose group was significantly increased （ P 〈 0.01 ）. Conclusion：XJP can regulate Walker256 rat thymus index, spleen index and bone marrow and thymus cell cycle. XJP can regulate T lymphocyte subsets CD4＋ , CD8＋ , CD4 ＋/CD8＋ ratio and NK cell activity. XJP with immune protective effect may be one of the mechanisms of its anti - tumor effects.

关 键 词：消癌解毒方 免疫功能 T淋巴细胞亚群 NK细胞 

分 类 号：R282.7[医药卫生—中药学]