川芎嗪对人胃癌细胞株SGC-7901/ADR增殖、凋亡和MDR1、GST-π表达的影响  

Effect of Tetramethylpyrazine on Proliferation and Apoptosis and Expressions of MDR1 and GST-π in Human Gastric Carcinoma Cell Line SGC-7901/ADR

作　　者：杨伟霞[1] 陆少锋[1] 贾宁[1] 赵冠耀[1] 

机构地区：[1]佛山市高明区人民医院药剂科,广东佛山528500

出　　处：《中华中医药学刊》2015年第8期2008-2010,I0033,I0034,共5页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的：探讨川芎嗪诱导人胃癌细胞株SGC-7901/ADR凋亡及对MDR1、GST-π表达的调控作用。方法：不同剂量（0~120μM）川芎嗪内酯与人胃癌细胞株SGC-7901/ADR同培养不同时间（24、48及72 h）,50μM 5-氟尿嘧啶（5-FU）作为阳性对照组,采用CCK8法测定细胞的增殖,流式细胞术测定细胞凋亡率。用不同剂量的川芎嗪与细胞培养48 h后,采用Western blot测定MDR1、GST-π蛋白的表达情况,用半定量RT-PCR测定MDR1、GST-πmRNA表达。结果：不同浓度川芎嗪处理人胃癌细胞株SGC-7901,川芎嗪抑制SGC-7901细胞增殖呈浓度及作用时间依赖性且120μM川芎嗪的抑制率与阳性对照组相当;而且应用流式细胞仪检测发现川芎嗪作用于人胃癌细胞株SGC-7901 48 h后,细胞出现凋亡。Western blot及半定量RT-PCR检测细胞中MDR1、GST-π及其mRNA的表达,结果显示,川芎嗪可下调MDR1、GST-π表达,下调作用与TMZ相当甚至更具优势。结论：川芎嗪能够抑制人胃癌细胞株SGC-7901的增殖,诱导其凋亡,且能抑制细胞中MDR1、GST-π的表达,其表达量与川芎嗪剂量呈现明显的时间-浓度效应关系。Objective ： To investigate the effect of tetramethylpyrazine on proliferation and apoptosis and MDR1 and GST -π expressions in human gastric carcinoma cell line SGC - 7901. Methods ： SGC - 7901 cells were treated with tet- ramethylpyrazine at different concentrations （0 ～ 100 μM） for 24,48 and 72 h,respectively. Cell proliferation was detec- ted by CCKS. The apoptosis rate of SGC - 7901 cells after treated with tetramethylpyr .azine for 48 h was evaluated by flow cytometry. The expressions of MDR1 ,GST-π and MDR1 ,GST-π mRNA were determined by Western blot and QRT- PCR, respectively. Results： Tetramethylpyrazine inhibited the proliferation of SGC -7901 cells and the inhibitory rates increased at a dose - time - dependent manner. Compared to the control group, the expressions of MDR1, GST -π and MDR1, GST -π mRNA in the treatment groups were significantly down - regulated. Conclusion ： Tetramethylpyrazine in- hibits proliferation and apeptosis in human gastric carcinoma cell line SGC -7901, which may be through the down- regulation of MDR1 and GST-π expressions in cells.

关 键 词：川芎嗪 人胃癌细胞株SGC-7901 MDR1 GST-Π 

分 类 号：R285.6[医药卫生—中药学]