一种制备结直肠癌原位移植瘤动物模型的方法  

A Method for Establishing Colorectal Orthotopic Transplantable Cancer in Mice

作　　者：杨喜裂 李耀平[2] 任连生[1] 赵莉莉[1] 杨永明[1] 阎磊[1] 白喜花[1] 

机构地区：[1]山西医科大学附属肿瘤医院比较医学部,太原030013 [2]山西医科大学附属肿瘤医院肛肠科,太原030013

出　　处：《实验动物与比较医学》2015年第4期305-307,共3页Laboratory Animal and Comparative Medicine

基　　金：山西省实验动物专项基金课题[2014（K03）]

摘　　要：目的设计一种制备小鼠肠原位移植瘤模型的新装置，并用其建立结直肠癌原位移植瘤动物模型。方法制备一种建立小鼠结直肠癌原位移植瘤的装置，使用该装置于KM小鼠结直肠原位接种1×10^6的H22肿瘤细胞，观察其成瘤情况及肿瘤转移情况。结果将H22细胞接种于昆明小鼠结直肠部位，小鼠平均生存时间为16．5±4．5d。动物死亡后解剖，观察到6只动物全部形成肿瘤，成瘤率为100％，个别动物有骼淋巴结转移，转移率为33.3％。结论采用自制结直肠癌原位接种器可成功建立结直肠癌原位移植瘤动物模型，该方法具有操作简便，成瘤率高的优点。Objective To develop a self-made inoculator for establishing orthotopic transplantable colorectal cancer in mice. Methods An inoculator for establishing orthotopic transplantable colorectal cancer in mice was designed and made. The inoculator was used for inoculating 1 × 10^6 H22 cells into colorectal site of each KM mice, and the tumor formation and metastasis was observed. Result Orthotopic transplanted H22 cells to colorectal site of Kunming mice, the tumor formation rate was 100%, the tumor metastasis rate was 33.3%, and the average lifetime of the mice was 16.5 ±4.5 d. Conclusion The self-made inoculator could be successfully used for establishing orthotopic transplantable colorectal cancer in mice. The operation was convenient, and the tumor formation rate was high.

关 键 词：结直肠癌 原位移植瘤 动物模型 

分 类 号：Q95-33[生物学—动物学]