银杏内酯B对视神经钳夹伤后大鼠视网膜神经节细胞的保护作用  

Protective Effect of Ginkgolide B on Retinal Ganglion Cells after Crush of Intra Orbital Optic Nerve in Rats

作　　者：郑幼平[1] 吴小桃[1] 田原[2] 张晓明[2] 周小娟[2] 孟晶[2] 

机构地区：[1]广州市番禺区中医院,广东广州511400 [2]暨南大学附属第一医院

出　　处：《中国医学创新》2015年第21期13-16,共4页Medical Innovation of China

基　　金：广东省医学科研基金(A2014627)

摘　　要：目的:探讨银杏内酯B对视神经钳夹伤后大鼠视网膜神经节细胞(RGCs)的保护作用。方法:取出生后42 d的SD大鼠36只,随机抽取12只为A组(正常对照组);24只分离暴露视神经并进行视神经钳夹后随机分为B组(模型组)和C组(GB治疗组),每组12只。每只鼠的右眼用于实验。A组不作任何处理,B、C组分别于实验前1周每日腹腔注射相应体积生理盐水和银杏内酯B(GB)40 mg/kg,术后继续给药1周,术后1、2周分别行闪光视觉诱发电位和HE染色光镜检查,并计数术后2周视网膜垂直经线RGCs数。结果:术后1周,A、B、C三组的LPl和APl分别为[(90±4)ms,(19±3)μV]、[(110±6)ms,(11±3)μV]和[(94±4)ms,(18±2)μV],B组与A、C组比较差异有统计学意义(P<0.05);术后2 w,A、B、C三组的LPl和APl分别为[(92±11)ms,(21±3)μV]、[(186±15)ms,(7±2)μV]和[(104±12)ms,(14±3)μV],RGCs数目分别为(281±39)个、(112±20)个、(228±35)个,各指标A、B、C三组比较差异均有统计学意义(P<0.05)。结论:银杏内酯B能抑制部分大鼠视神经钳夹后RGCs凋亡,具有一定的视神经保护作用。Objective：To investigate the protective effect of ginkgolide B on retinal ganglion cells after crush of intra orbital optic nerve in rats.Method：Thirty-six SD rats which were 46-day-old were randomly divided into noral control group（A group,12 rats）,model control group（B group,12 rats）and Ginkgolide B group（C group,12 rats）.In each group,only the right eyes were used.No operation was carried out in normal control group,corresponding volume of NS or Ginkgolide B at 40 mg/kg was injected intraperitoneally into the rats from one week before crush until one week after crush in model control group or Ginkgolide B group,one week and two weeks after operation,flash visual evoked potential were performed.Two weeks after operation,calculated the number of RGCs in each group.Result：one week after operation,the coefficients of LPl and APl were[（90±4）ms,（19±3）μV],[（110±6）ms,（11±3）μV]and[（94±4）ms,（18±2）μV]respectively in noral control group,model control group and Ginkgolide B group,the differences were statistically significant among B group and A group or C group（P〈0.05）.Two weeks after operation,the coefficients of LPl and APl were[（92±11）ms,（21±3）μV],[（186±15）ms,（7±2）μV]and[（104±12）ms,（14±3）μV] respectively,the number of RGCs were（281±39）,（112±20）and（228±35）respectively,the differences were statistically significant in 3 groups（P〈0.05）.Conclusion：Ginkgolide B can partly inhibit the apoptosis of RGCs and promote the survival of RGCs after crush of intra orbital optic nerve.

关 键 词：银杏内酯B 视神经损伤 视网膜神经节细胞 F-VEP 大鼠 

分 类 号：R285.5[医药卫生—中药学]