电针对实验性兔膝骨关节炎病变软骨BMP-2/Smad1表达的影响  

Effects of electroacupuncture on regulating the expressions of BMP2/Smad1 in impaired cartilage of experimental rabbits with knee osteoarthritis

作　　者：邵湘芝 陶红星[1] 杨万章[1] 李健[2] 滕斌[2] 任培根[2] 

机构地区：[1]深圳市南山区人民医院,深圳518052 [2]中国科学院深圳先进技术研究院

出　　处：《中国康复医学杂志》2015年第7期655-660,666,共7页Chinese Journal of Rehabilitation Medicine

基　　金：深圳市南山区人民医院科研基金资助项目(2013005)

摘　　要：目的:观察电针(EA)治疗兔膝骨关节炎(KOA)模型关节软骨中骨形态发生蛋白-2(BMP-2)、Smad1表达的变化,探讨电针治疗KOA的作用机制。方法:制作兔膝关节炎模型,将建模成功的新西兰兔纳入模型对照组;正常组和模型组行常规饲养,不行治疗;电针组行电针治疗20d。实验结束后,分别观察各组兔行为学评分、右膝关节宽度、软骨大体形态及组织学评分、软骨BMP-2、Smad1免疫组化及实时荧光定量PCR的变化。结果:电针组与模型组在行为学评分、软骨大体形态及组织学评分(Mankin评分)方面差异均无显著性(P>0.05),电针组兔右膝关节宽度小于模型组(P<0.05)。各组BMP-2免疫组化结果无显著差异,电针组较模型组Smad1蛋白质表达降低。模型组和模型对照组BMP-2 m RNA表达水平较正常组上调,差异无显著性意义(P>0.05),模型组Smad1m RNA表达水平较模型对照组和正常组均显著上调(P<0.05),电针组BMP-2及Smad1 m RNA表达水平较模型对照组和模型组均显著下调(P<0.05)。结论:电针可能通过下调早期兔膝骨关节炎软骨BMP-2/Smad1的表达抑制骨赘形成,延缓膝骨关节炎的病理进程。Objective：To explore the mechanism about electroacupuncture（EA） treatment of knee osteoarthritis（KOA） by ob- serving the expressions of BMP2, Smadl in impaired cartilage of rabbits with KOA after EA treatment. Method：Models of KOA were established with papain.The model control group had successfully modeled rab- bits.The treatment group was treated with EA for 20 days. The normal group and model group were bred with- out treatment.Behavior scoring,right knee width,cartilage morphology, Mankin score and the expressions of BMP- 2,Smadl using immunohistochemical and real-time PCR methods were analysised. Result：The treatment group and model group showed no significant difference in behavior scoring,cartilage mor- phology and Mankin score（P〉0.05）.The treatment group showed significant smaller right knee width compared with the model group（P〈0.05）.No significant difference of BMP-2 protein level were showed between two groups.The treatment group showed lower Smadl protein level compared with the model group.The model group and model control group showed no significant higher level of BMP-2 mRNA compared with the nor- mal group（P〉0.05）.The model group showed higher levels of Smadl mRNA compared with the model controlgroup and normal group（P〈0.05）.The treatment group Smadl mRNA compared with the model control group Conclusion： The EA showed therapeutical effect, could 2, and Smadl levels. showed significant lower levels of BMP-2 mRNA and and model group（P〈0.05）. delay the development of rabbit KOA by reducing BMP-2, and Smadl levels.

关 键 词：电针 膝骨关节炎 软骨修复 BMP-2 SMAD1 骨赘形成 

分 类 号：R245[医药卫生—针灸推拿学]