佐剂关节炎大鼠滑膜组织HIF-1α和VEGF及CD34表达上调促进血管新生  

Up-regulated expressions of HIF-1α,VEGF and CD34 promote synovial angiogenesis in rats with adjuvant arthritis

作　　者：张晓军[1] 刘健[2] 万磊[2] 孙玥[2] 王芳[2] 齐亚军[1] 黄传兵[2] 

机构地区：[1]安徽中医药大学,安徽合肥230038 [2]安徽中医药大学第一附属医院,安徽合肥230031

出　　处：《细胞与分子免疫学杂志》2015年第8期1053-1056,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81173211);国家科技支撑计划课题(2012BA126B02);国家中医药重点学科中医痹病学建设项目(国中医药发[2009]30号);安徽省自然科学基金(1208085MH180);安徽省科技厅科研计划项目(09020304046);安徽省卫生厅中医药科研项目(2009ZY05);安徽中医学院科技创新团队项目(2010TD005);安徽中医药大学校级课题(2010LC-020)

摘　　要：目的观察佐剂关节炎大鼠滑膜组织缺氧诱导因子1α(HIF-1α)、血管内皮细胞生长因子(VEGF)及CD34的表达,探讨类风湿性关节炎血管新生的机制。方法 30只大鼠随机均分为正常对照组和模型对照组,模型对照组采用Freund完全佐剂建立佐剂关节炎大鼠模型。造模成功后19 d,采用反转录PCR检测滑膜组织HIF-1α、VEGF的mRNA表达,Western blot法检测HIF-1α、VEGF的蛋白表达,免疫组织化学染色检测大鼠肺组织HIF-1α、VEGF、CD34表达。结果与正常对照组比较,模型对照组关节炎症明显,滑膜组织CD34表达增加,VEGF、HIF-1αmRNA和蛋白表达显著增加;滑膜组织VEGF mRNA表达与足趾肿胀度呈正相关,HIF-1α蛋白表达与关节炎指数呈正相关,VEGF mRNA、HIF-1α蛋白表达量与CD34呈正相关。结论佐剂关节炎大鼠滑膜组织VEGF、HIF-1α、CD34过表达与滑膜血管新生有关。Objective To observe the alterations of hypoxia inducible factor 1α（HIF-1α）,vascular endothelial growth factor（ VEGF） and CD34 in synovial tissues in rats with adjuvant arthritis and explore the mechanism of angiogenesis in rheumatoid arthritis. Methods Thirty SD rats were randomly divided into normal control and model groups. Adjuvant arthritis was induced using Freund ＇s complete adjuvant in the rats of the model group. Nineteen days after the modeling,the expressions of HIF-1α,VEGF and CD34 were detected using immunohistochemistry. The mRNA and protein levels of HIF-1α and VEGF were detected by reverse transcription PCR and Western blotting,respectively. Results Compared with the normal control group,the model group showed paw swelling,increased arthritic index,and significantly up-regulated expressions of VEGF,HIF-1α,CD34 in synovial tissue. VEGF mRNA was positively correlated with paw swelling degree,HIF-1α protein was positively correlated with the arthritis index,and VEGF mRNA and HIF-1α protein were positively correlated with CD34.Conclusion The synovial angiogenesis is associated with the over-expressions of VEGF,HIF-1α and CD34 in rats with adjuvant arthritis.

关 键 词：佐剂关节炎 血管新生 缺氧诱导因子1α 血管内皮细胞生长因子 CD34 

分 类 号：R684.3[医药卫生—骨科学] R392-33[医药卫生—外科学]