过表达Src羧基末端激酶结合蛋白的Jurkat细胞荷瘤小鼠模型的建立与鉴定  

Establishment and identification of Jurkat cell xenograft mouse models with over-expression of C-terminal Src kinase binding protein

作　　者：高美华[1] 马宇[1] 王冰[1] 张蓓[1] 张敏锐[1] 张树超[2] 

机构地区：[1]青岛大学医学院免疫学教研室 [2]青岛大学医学院附属医院,山东青岛266071

出　　处：《细胞与分子免疫学杂志》2015年第9期1167-1169,1174,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81273206)

摘　　要：目的建立过表达Src羧基末端激酶结合蛋白(CBP)的T系白血病Jurkat细胞动物模型。方法 5周龄雌性BALB/c-nu小鼠随机分为空白对照组、正常Jurkat细胞对照组、空病毒Jurkat细胞对照组、病毒转染过表达CBP模型组,每组5只。小鼠腋窝皮下注射Jurkat细胞1×107个/0.1 m L。建模成功后取出完整瘤体组织称质量、HE染色观察小鼠皮下肿块的病理变化;流式细胞术检测小鼠外周血Jurkat细胞增殖水平;ELISA检测小鼠血清中白细胞介素2(IL-2)水平。结果病毒转染过表达CBP模型组小鼠瘤体组织块体积和质量小于对照组,HE染色瘤体内有Jurkat细胞增殖,外周血Jurkat细胞增殖水平低于正常Jurkat细胞对照组和空病毒Jurkat细胞对照组,血清中IL-2水平低于正常Jurkat细胞对照组和空病毒Jurkat细胞对照组。结论成功建立了过表达CBP的Jurkat白血病细胞小鼠荷瘤模型,CBP可抑制Jurkat细胞IL-2分泌和增殖。Objective To establish xenograft mouse models of Jurkat T-leukemia cells over-expressing C-terminal Src kinase binding protein（ CBP）. Methods The 5-week-old female BALB / c-nu mice were randomly divided into blank control group,normal Jurkat cell control group,empty virus-transfected Jurkat cell control group and CBP over-expression model group,5 mice in each group. The mice were subcutaneously injected 1 × 107/0. 1 m L Jurkat cells in axillary area. The tumor tissues of mouse models were weighed and then subjected to HE staining to observe the pathological changes of tumor tissues. The proliferation of Jurkat cells in the peripheral blood of mice was detected by flow cytometry,and the interleukin 2（IL-2） levels in mouse sera were determined with ELISA. Results The volume of tumor tissues in the CBP over-expression model group was smaller than that in the control groups,so was the mass of tumor tissues. HE staining showed the proliferation of Jurkat cells in tumor tissues of the model group. The proliferation rate of Jurkat cells in the peripheral blood and IL-2 levels in the sera of the CBP over-expression model group were lower than those in the normal Jurkat cell control group and the empty virus-transfected Jurkat cell control group. Conclusion The mouse models of Jurkat T-leukemia cells over-expressing CBP have been established successfully. Up-regulated CBP has inhibitory effects on the proliferation of Jurkat cells and IL-2secretion.

关 键 词：Src羧基末端激酶结合蛋白(CBP) T系白血病 JURKAT细胞 小鼠模型 

分 类 号：R392.12[医药卫生—免疫学] R733.7[医药卫生—基础医学]