Cytocompatible 3D chitosan/hydroxyapatite composites endowed with antibacterial properties:toward a self-sterilized bone tissue engineering scaffold  

作　　者：Yongjun Qiao Zhongjun Zhai Limei Chen Hong Liu 

机构地区：[1]Industry Group of Shandong University [2]State Key Laboratory of Crystal Materials, Shandong University [3]Innowit Co., Ltd

出　　处：《Science Bulletin》2015年第13期1193-1202,共10页科学通报（英文版）

基　　金：supported by the National Natural Science Foundation of China(51372142,51321091);the Fundamental Research Funds of Shandong University(2014QY003-09)

摘　　要：Bone tissue scaffolds based on bioactive polymer–hydroxyapatite composites have caused infections that seriously limit their extended application. In this study, we proposed a practical ion substitution method to synthesize in situ silver phosphate on the surface of a two-level, threedimensional chitosan/nano-hydroxyapatite scaffold. A release test of silver ions in a phosphate buffered saline(PBS) solution was performed to demonstrate that silver ions were released continuously from the silver phosphate during the initial 6 days of the study. The antibacterial property and cytocompatibility of the scaffolds treated with different concentrations of silver nitrate solution were assessed by in vitro assays with Escherichia coli and MC3T3-E1, respectively. The ability of the silver-containing scaffolds to induce bacteriostasis was confirmed by the inhibition zone(15 mm) and high bactericidal rate([99 %). Cell proliferation, morphology and the alkaline phosphatase activity of MC3T3-E1 cultured on the scaffold with low silver phosphate contents were comparable with those cultured on control samples.Bone tissue scaffolds based on bioactive poly- mer-hydroxyapatite composites have caused infections that seriously limit their extended application. In this study, we proposed a practical ion substitution method to synthesize in situ silver phosphate on the surface of a two-level, threedimensional chitosan/nano-hydroxyapatite scaffold. A release test of silver ions in a phosphate buffered saline （PBS） solution was performed to demonstrate that silver ions were released continuously from the silver phosphate during the initial 6 days of the study. The antibacterial property and cytocompatibility of the scaffolds treated with different concentrations of silver nitrate solution were assessed by in vitro assays with Escherichia coli and MC3T3-E1, respectively. The ability of the silver-containing scaffolds to induce bacteriostasis was confirmed by the inhibition zone （15 mm） and high bactericidal rate （〉99 %）. Cell proliferation, morphology and the alkaline phosphatase activity of MC3T3-E1 cultured on the scaffold with low silver phosphate contents were comparable with those cultured on control samples.

关 键 词：Silver phosphate . Ion substitution .Chitosan/nano-hydroxyapatite bone scaffold .Bacteriostasis 

分 类 号：R318.08[医药卫生—生物医学工程] TB332[医药卫生—基础医学]