创伤程度与肿瘤生长关系的实验研究  

The experimental study of relationship between trauma severity and tumor growth

作　　者：邱宝安[1] 曹卫刚[1] 涂亮[1] 赵文超[1] 夏念信[1] 杨英祥[1] 祝建勇[1] 安阳[1] 吴印涛[1] 

机构地区：[1]海军总医院肝胆外科,北京100048

出　　处：《中华肝胆外科杂志》2015年第7期482-485,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：北京市科委“首都市民健康培育项目”（Z141100002114015）

摘　　要：目的探讨全身性创伤与肿瘤生长的关系。方法选用Wistar大鼠36只，Wistar幼鼠1只；以烫伤作为全身性创伤的致伤方法，将大鼠分为三组，分别为对照组、轻度烫伤组及中度烫伤组，每组12只。幼鼠用于Walker-256细胞株腹水瘤传代保种。三组大鼠用幼鼠腹腔培养的肿瘤细胞建立左侧腹股沟移植瘤模型，10天后轻度烫伤组及中度烫伤组于背部分别致10％和15％烫伤，不同时间采血并于14天后采集组织标本。观察不同时间的皮质醇、IL-1β及TNF—α变化。对肿瘤组织行组化检查，观察血管内皮生长因子（VEGF）和增殖细胞核抗原（PCNA）变化。结果大鼠组织烫伤后，皮质醇、IL-18及TNF-α均较对照组升高，且呈进行性升高，差异有统计学意义。2周取肿瘤检查，肿瘤大小变化显示对照组（0．593Cm3±0．195cm3）与轻伤组（0．782cm3±0．344cm3）肿瘤体积，无统计学差异，中度烫伤组（1．006cm3±0．838cm3）肿瘤体积明显大于对照组和轻度烫伤组（P〈0．05）。肿瘤组织免疫组化显示对照组、轻度烫伤组及中度烫伤的大鼠肿瘤组织中PCNA的表达分别为58．3％±6．6％、77．1％±4．5％和81．6％±d．6％，以中度烫伤变化为大，差异有统计学意义。同时，轻度及中度烫伤组VEGF阳性例数明显高于对照组，差异有统计学意义（P〈0．05）。结论烫伤所致不同程度组织创伤反应可引起机体不同炎症介质的释放以及肿瘤组织周围VEGF和PCNA的增加，从而导致肿瘤组织生长速度的改变，说明全身性创伤与肿瘤的生长存在联系。Objective To explore the relationship between systemic trauma and tumor growth. Methods 36 Wistar rats with systemic trauma caused by scald were randomly divided into three groups as control group, mild-scald group and moderate-scald group. And one Wistar pup was used for ascite tumor cell growth （Walker-256 line） which offered to plant the tumor in each groups. The scald was made in the back 10 days after tumor plant with different degrees （10% and 15% ）. Blood samples were collected at dif- ferent time and tumor tissues were harvested after 14 days. laboratory studies including cortisol, IL-1β and TNF-α by ELISA. Vascular endothelial growth factor （VEGF） and proliferating cell nuclear antigen （PCNA） were observed in Tumor tissues through histochemistry assay. Results The levels of cortisol, IL- lβ and TNF-aelevated significantly from control group to mild scald group and moderate scald group. Con- cerning the tumor growth, the size of tumor in control group and mild scald group were （0. 593 ± 0. 195 ）cm3 and （0. 782 ± 0. 344）cm3 respectively without statistic differences, but the size of moderate scald group was （ 1. 006 ± 0. 838 ） em3 which had significant difference compared to the first two groups. The histochemieal studies showed the expression of PCNA in tumor tissues of control group, mild scald group and moderate scald group were 58.3% ±6. 6%, 77. 1% ±4. 5% and 81.6% ±4. 6% with significant difference. And the expression of VEFG in tumor tissue showed the same results as PCNA expression. Conclusions Different degree of scald may cause different severity of traumatic tissue response which could lead to different release of inflammatory mediators and the increase of VEGF and PCNA in tumor tissue. Furthermore all those chan- ges may influence the tumor growth which suggests that systemic trauma has some relationship with tumor growth.

关 键 词：创伤 肿瘤 增生 

分 类 号：R730.2[医药卫生—肿瘤]