高脂血症大鼠肝脏中牛磺胆酸钠转运蛋白的表达状况  

Na^+/taurocholate cotransporting polypeptide expression in liver tissue of hyperlipidemia rats

作　　者：张孟瑜[1] 王洁萍[2] 夏先明[1] 

机构地区：[1]泸州医学院附属医院肝胆外科,四川省泸州市646000 [2]泸州医学院附属医院康复科,四川省泸州市646000

出　　处：《世界华人消化杂志》2015年第19期3123-3128,共6页World Chinese Journal of Digestology

摘　　要：目的:本研究以牛磺胆酸钠转运蛋白(Na+/taurocholate cotransporting polypeptide,Ntcp)为研究对象,通过分析Ntcp在高脂血症大鼠模型肝脏中的表达状况,寻求新的高脂血症治疗措施.方法:取♂Wistar大鼠(体质量150 g±5 g)60只,随机均分为实验组及对照组.喂食90 d,建立高脂血症模型,喂食期间定期取血检测胆固醇及胆汁酸含量,建模成功后,分别取两组大鼠肝脏组织.应用逆转录-聚合酶链反应(RT-polymerase chain reaction,RTP C R)技术检测肝脏组织的N t c p基因表达的状况.应用免疫组织化学SP(streptavidinperosidase)法检测肝脏组织的Ntcp蛋白表达的状况.结果:实验组胆固醇及胆汁酸含量提高明显.电泳结果显示:两组肝脏组织都出现了位于425 bp的内参β-actin基因的扩增带和位于325 bp的Ntcp基因扩增带,实验组Ntcp基因扩增带亮度减弱;免疫组织化学结果显示:实验组Ntcp表达阳性率为23.6%,对照组N t c p表达阳性率为75.2%,差异有统计学意义(χ2=9.858,P<0.05).结论:实验组大鼠的Ntcp基因的表达明显减弱,提示我们Ntcp基因可能会成为高脂血症及其相关疾病新的药物治疗靶点.AIM：To establish a rat model of hyperlipidemia,detect the expression of Na＋/taurocholate cotransporting polypeptide（Ntcp） in this model,and explore the role of Ntcp in bile acid and cholesterol metabolism in hyperlipidemia rats.METHODS：Sixty Wistar rats were randomly divided into two groups,a control group（control group） that was fed an ordinary diet,and a high fat diet group（experimental group） that was fed a high fat diet.Regular detection of cholesterol and bile acid content was conducted to assess whether the hyperlipidemia model was successfully established.After successful induction of hyperlipidemia,liver tissues were taken to detect the m RNA expression of Ntcp by reverse transcription-polymerase chain reaction（RT-PCR） and protein expression by immunohistochemistry（streptavidinperoxidase）.RESULTS：In the experiment group the contents of cholesterol and bile acid were significantly higher than those in the control group.RT-PCR analysis showed that the mR NA expression of Ntcp in the liver tissue was significantly lower in the experimental group than in the control group.Immunohistochemistry showed that in the experimental group,the positive expression rate of Ntcp was 23.6%,significantly lower than 75.2% in the control group（χ2 = 9.858,P 0.05）.CONCLUSION：Ntcp expression decreases significantly in rats with hyperlipidemia,suggesting that Ntcp may be used as a target for treatment of hyperlipidemia and related diseases.

关 键 词：牛磺胆酸钠转运蛋白 高脂血症 WISTAR大鼠 

分 类 号：R589.2[医药卫生—内分泌]