EV71受体SCARB2和PSGL-1在重症手足口病患者肺组织中的表达  

Expression and location of EV71 receptors SCARB2 and PSGL-1 in lung tissues of fatal hand,foot and mouth disease

作　　者：姚莎[1] 潘红波[1] 侯碧海[2,3] 党裔武[1] 李坤雄[1] 陈罡[1] 罗殿中[1] 

机构地区：[1]广西医科大学第一附属医院病理科,南宁530021 [2]广西警官高等专科学校,南宁530023 [3]广西金桂司法鉴定中心,南宁530022

出　　处：《临床与实验病理学杂志》2015年第7期780-783,共4页Chinese Journal of Clinical and Experimental Pathology

基　　金：广西医疗卫生重点科研课题(2011097);广西自然科学基金(2013GXNSFAA019157)

摘　　要：目的探讨人肠道病毒71型(enterovirus 71,EV71)清道夫受体B2(scavenger receptor class B member 2,SCARB2)和人P选择素糖蛋白配体-1(P-selectin glycoprotein ligand-1,PSGL-1)在重症手足口病(hand,foot and mouth disease,HFMD)患者、正常儿童及成人肺组织中的定位和分布。方法运用免疫组化Super Vision两步法检测15例重症HFMD患者、3例正常儿童及8例成人肺组织中SCARB2和PSGL-1的表达及分布。结果 SCARB2分布于重症HFMD患者、正常儿童及成人肺组织支气管上皮、细支气管上皮、肺泡上皮细胞及炎症细胞,其在三组中的阳性率差异无显著性(P>0.05)。PSGL-1分布于成人肺组织支气管、细支气管上皮,但在重症HFMD患者及正常儿童中不表达,阳性率分别为100%、0、0,三组比较差异均有统计学意义(P<0.05),三组肺组织的炎症细胞PSGL-1均为阳性,阳性率分别为100%、66.7%、100%(P>0.05);PSGL-1在三组的肺泡上皮细胞中均不表达。结论 EV71受体SCARB2分布于重症HFMD患者肺组织支气管上皮、细支气管上皮、炎症细胞及肺泡上皮细胞,PSGL-1仅分布于重症HFMD患者肺组织炎症细胞,提示SCARB2可能在重症HFMD的感染过程中具有一定作用。Purpose To investigate the location and distribution of EV71 receptors scavenger receptor class B member 2 （ SCARB2 ） and human P-selectin glycoprotein ligand-1 （PSGL-1） in lung tissues of fatal hand, foot and mouth disease （HFMD）, healthy children and adults. Methods The expression of EV71 receptors SCARB2 and PSGL-1 was detected by using immunohistochemistry in lung tissues of 15 autopsies of HFMD, 3 of healthy children, 8 of healthy adults. Results SCARB2 distributed in bronchial, bronchioli ep-ithelia, alveolar epithelial cells and inflammatory cells among HFMD, healthy children and adults. No significant difference was noted of the positive rates of SCARB2 expression among these three groups （P〉0. 05）. PSGL-1 distributed in bronchial and bronchioli epi-thelium of adults, but no PSGL-1 expression was found in HFMD and healthy children. The positive rates of PSGL-1 were 100%, 0, 0 in bronchial and bronchioli epithelium among the three groups, respectively （P〈0. 05）. The positive rates of PSGL-1 were 100%, 66. 7%, 100% in inflammatory cells among HFMD, healthy children and adults, respectively. No significant difference was noted of PSGL-1 expression among the three groups （P〉0. 05）. Further, no PSGL-1 expression was observed in alveolar epithelia cells of all groups tested. Conclusions EV71 receptor SCARB2 distributes in bronchial, bronchioli, alveolar epithelial and inflammatory cells of HFMD. Meanwhile, PSGL-1 only distributes in inflammatory cells of HFMD, suggesting that SCARB2 possibly plays a role on HFMD infection.

关 键 词：手足口病 肠道病毒71型 清道夫受体B2 P选择素糖蛋白配体-1 免疫组织化学 

分 类 号：R725.1[医药卫生—儿科]