煤焦沥青职业接触人群DNA甲基化和端粒损伤研究  

DNA methylation and telomere damage in occupational people exposed to coal tar pitch

作　　者：王艳斌[1] 段晓冉 张育红 王思华 姚武[2] 王诗斌[1] 王威[2] 吴拥军[2] 

机构地区：[1]安阳钢铁股份有限公司安全管理处劳动保护科,455000 [2]郑州大学公共卫生学院职业卫生与职业医学教研室 [3]郑州市职业病防治院 [4]河南省职业病防治研究院

出　　处：《中华劳动卫生职业病杂志》2015年第7期507-511,共5页Chinese Journal of Industrial Hygiene and Occupational Diseases

基　　金：国家自然科学基金（30972457,81001239）;河南省重大科技攻关项目（112102310102）;河南省医学科技攻关计划项目（2011020082）

摘　　要：目的研究煤焦沥青职业接触人群p16、FHIT和RASSF1A基因启动子甲基化及端粒损伤情况，探讨煤焦沥青职业接触有效的生物标志。方法选择某碳素厂180例煤焦沥青职业接触工人为接触组，同期在郑州大学第一附属医院进行体检的健康人145例为对照组。用实时荧光定量PCR的方法检测外周血DNA相对端粒长度，实时荧光定量甲基化特异性PCR方法检测外周血DNAp16、RASSF1A和FHIT基因甲基化率，比较两组端粒相对长度和基因甲基化率情况并分析影响因素。结果接触组外周血白细胞DNA端粒相对长度[0．83（0．71～0．90）]低于对照组端粒相对长度[1．13（0．72～1．45）]，差异有统计学意义（Z=-5．395，P〈0．01）。两组p16、FHIT和RASSF1A基因启动子甲基化率的差异无统计学意义（P〉0．05）。经过性别、年龄和是否吸烟的分层分析后发现，在年龄≤40岁时接触组p16基因启动子的甲基化率高于对照组，差异均有统计学意义（Z=-1．914，P=0．011）。结论煤焦沥青职业接触可引起人外周血白细胞DNA端粒长度缩短，对p16、FHIT和RASSF1A基因启动子甲基化率无影响。Objective To investigate the promoter methylation of p16, FHIT and RASSF1A gene and telomere damage in the workers exposed to coal tar pitch, and to explore the effective biomarker of occupational exposure to coal tar pitch. Methods 180 eases of workers exposed to coal tar pitch in a certain carbon plant named as exposure group, and 145 healthy eases with a medical examination in the first affiliated hospital of Zhengzhou University were selected as control group. Relative telomere length in peripheral blood DNA was detected using real-time quantitative PCR, and the promoter methylation rate of p16, RASSF1A and FHIT gene in peripheral blood DNA were determined by real-time quantitative methylation specific PCR. The relative telomere length and gene promoter methylation in two groups were compared, and influencing factors were analyzed. Results Relative telomere length in exposed group was lower than that in the control group, and the difference was statistically significant （Z=-5.395, P〈0.001 ）. There was no significant difference in the promoter methylation rate of p16, FHIT and RASSF1A gene between the two groups （P〉0.05）. Stratification analysis by gender, age, and smoking, we found that when the age was less than or equal to 40, the promoter methylation rate of p16 in exposed group was more than that in control group, and the difference was statistically significant （Z=-1.914, P=0.011）. Conclusion Occupational exposure to coal tar pitch may induce leukocyte DNA telomere length of human peripheral blood shortened, and may not change the promoter methylation rates of p16, FHIT and RASSF1A gene.

关 键 词：煤焦沥青 DNA甲基化 端粒 

分 类 号：R131[医药卫生—劳动卫生]