Establishment, characterization, and application of pAcr-SP-NTP-EGFP transgenic mice in visualizing the oviduct-migrating ability of sperm from Prss37-null mice  

作　　者：Chunling Shen Jinjin Wang Hua Zhuang Jianbing Liu Xiyi Wang Xuejiao Chen Zhuanbin Wu Wenting Wu Youbing Wu Yanwen Sun Huimin Yan Jian Fei Ying Kuang Zhugang Wang 

机构地区：[1]State Key Laboratory of Medical Genomics, Research Center for Experimental Medicine, Shanghai Ruijin Hospital Affiliated to Shanghai Jiao Tong University School of Medicine (SJTUSM), Shanghai 200025, China [2]2Shanghai Research Center for Model Organisms, Shanghai 201203, China [3]Model Organism Division, E-Institutes of Shanghai Universities, SJTUSM, Shanghai 200025, China

出　　处：《Acta Biochimica et Biophysica Sinica》2015年第6期466-473,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China （No. 81430028）, Ministry of Science and Technology of China （No. 2011BA115B02）, Science and Technology Commission of Shanghai Municipality （Nos. 13DZ2293700, 13140901400, and 13DZ2280600）, and E-Institutes of Shanghai Municipal Education Commission （No. E03003）.

摘　　要：Transgenic mouse model with fluorescently labeled sperm has extensive application value. It is an auxiliary tool for investigating the mechanism of fertilization, especially for visualizing the oviductmigrating ability of sperm in vivo. Here, we produced transgenic mouse lines whose sperm were tagged with enhanced green fluorescent protein （EGFP） according to the previously described method. Polymerase chain reaction analysis of tail-tip genomic DNA identified 13 founders, of which 5 male founders produced offspring to form transgenic lines. We showed that EGFP was testis-specifically expressed, sharing similar expression pattern with endogenous acrosin. It has luminal side restricted distribution in seminiferous tubules and acrosomal aggregation in mature sperm. In addition, interstrain hybridization obtained Prss37^-/-EGFP^tg/＋ males produced sperm with impaired oviduct-migrating ability as visualized under fluorescence microscope, compared with Prss37^＋/＋EGFP^tg＋ counterparts. These results indicate that a transgenic mouse model with fluorescently labeled sperm has been successfully established and it is a useful tool for evaluating the oviduct-migrating ability of sperm.Transgenic mouse model with fluorescently labeled sperm has extensive application value. It is an auxiliary tool for investigating the mechanism of fertilization, especially for visualizing the oviductmigrating ability of sperm in vivo. Here, we produced transgenic mouse lines whose sperm were tagged with enhanced green fluorescent protein （EGFP） according to the previously described method. Polymerase chain reaction analysis of tail-tip genomic DNA identified 13 founders, of which 5 male founders produced offspring to form transgenic lines. We showed that EGFP was testis-specifically expressed, sharing similar expression pattern with endogenous acrosin. It has luminal side restricted distribution in seminiferous tubules and acrosomal aggregation in mature sperm. In addition, interstrain hybridization obtained Prss37^-/-EGFP^tg/＋ males produced sperm with impaired oviduct-migrating ability as visualized under fluorescence microscope, compared with Prss37^＋/＋EGFP^tg＋ counterparts. These results indicate that a transgenic mouse model with fluorescently labeled sperm has been successfully established and it is a useful tool for evaluating the oviduct-migrating ability of sperm.

关 键 词：gene expression EGFP transgenic/knockout model oviduct-migrating ability 

分 类 号：Q78[生物学—分子生物学] Q959.5