难治性支气管哮喘患者血清肿瘤坏死因子α水平变化及其临床意义  

Changes and Clinical Significance of Serum TNF- α Level in Patients with Refractory Asthma

作　　者：林茂华[1] 王智辉[1] 唐雨晴[1] 胡春荣[1] 

机构地区：[1]广东省惠州市博罗县人民医院呼吸内科,516100

出　　处：《实用心脑肺血管病杂志》2015年第6期51-52,57,共3页Practical Journal of Cardiac Cerebral Pneumal and Vascular Disease

摘　　要：目的观察难治性支气管哮喘患者血清肿瘤坏死因子α(TNF-α)水平变化,并探讨其临床意义。方法选取2012年2月—2013年1月惠州市博罗县人民医院收治的支气管哮喘患者68例,根据哮喘诊断标准分为难治性哮喘患者34例(DTA组)和普通哮喘患者34例(非DTA组),另选取同期体检健康者34例(对照组)。采用酶联免疫吸附试验测定血清TNF-α及白介素6(IL-6)水平,自动血细胞分析仪测定嗜酸粒细胞直接计数(EOS),肺功能测试仪测定第一秒用力呼气容积(FEV1)。比较3组患者血清TNF-α、IL-6水平及EOS、FEV1,并分析血清TNF-α水平与血清IL-6水平、EOS及FEV1的相关性。结果 DTA组、非DTA组患者血清TNF-α、IL-6水平及EOS高于对照组,FEV1低于对照组(P<0.05)。DTA组患者血清TNF-α、IL-6水平及EOS高于非DTA组,FEV1低于非DTA组(P<0.05)。Pearson相关分析结果显示,血清TNF-α水平与血清IL-6水平、EOS呈正相关(r值分别为0.725、0.837,P值分别为0.017、0.003),与FEV1呈负相关(r=-0.742,P=0.012)。结论难治性支气管哮喘患者血清TNF-α水平明显升高,且与FEV1呈负相关,与血清IL-6水平、EOS呈正相关,临床上可将TNF-α阻断剂作为难治性支气管哮喘的治疗药物之一。Objective To observe the changes of serum TNF- α level in patients with refractory asthma,to investigate its clinical significance. Methods From February 2012 to January 2013 in the People＇s Hospital of Boluo County,a total of 68 patients with asthma were selected,and they were divided into A group（ with refractory asthma,n = 34） and B group（ with ordinary asthma,n = 34） according to asthma diagnosis standard; a total of 34 healthy cases were selected as control group at the same time. ELISA was used to detect the serum levels of TNF- α and IL-6,automatic hemocyte analyzer was used to detect eosinophilic granulocyte direct count（ EOS）,pulmonary function test instrument was used to detect FEV1. Serum levels of TNF- α and IL-6,EOS and FEV1 were compared among the three groups; correlations between serum TNF- α level and serum IL-6 level,EOS,FEV1 were analyzed. Results Serum levels of TNF- α and IL-6,EOS of groups A and B were statistically significantly higher than those of control group,while FEV1 of groups A and B was statistically significantly lower than that of control group,respectively（ P〈0. 05）. Serum levels of TNF- α and IL-6,EOS of A group were statistically significantly higher than those of B group, while FEV1 of A group was statistically significantly lower than that of B group（ P〈0. 05）.Pearson correlation analysis showed that,serum TNF- α level was positively correlated with serum IL-6 level（ r = 0. 725,P =0. 017）,EOS（ r = 0. 837, P = 0. 003）, respectively; was negatively correlated with FEV1（ r =- 0. 742,P = 0. 012）.Conclusion Serum TNF- α level is obviously increased in patients with refractory asthma,and it is negatively correlated with FEV1,positively correlated with serum IL-6 level and EOS,so TNF- α blockers can be used in the treatment of refractory asthma.

关 键 词：哮喘 肿瘤坏死因子Α 

分 类 号：R562.25[医药卫生—呼吸系统]