早期稳定型动脉粥样硬化斑块模型兔损伤区拉曼光谱特征及旋转手法的影响  

Influence of rotation manipulation on Raman spectral characteristics of stable plaques in a rabbit model of early carotid atherosclerosis

作　　者：谌祖江[1] 黄学成[1] 向孝兵 陈超[1] 李义凯[1] 

机构地区：[1]南方医科大学中医药学院,广东省广州市510515 [2]广州市正骨医院运动医学科,广东省广州市510045

出　　处：《中国组织工程研究》2015年第27期4339-4344,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金(81273871;30700893);广东省科技计划项目(2012B031800323)~~

摘　　要：背景:研究普遍认可颈椎旋转手法可增加颈动脉粥样硬化不稳定型斑块脱落的风险,然而该手法对颈动脉粥样硬化早期稳定型斑块的影响研究甚少。目的:探索颈椎旋转手法对动脉粥样硬化斑块模型兔脂质含量的影响。方法:将30只雄性新西兰兔经普通饲料喂养15 d后,随机选取10只作为对照组,继续以普通饲料喂养至18周。剩余20只改用含2%胆固醇、10%猪油和88%普通颗粒饲料的高脂饲料喂养至18周,建立早期稳定型动脉粥样硬化斑块动物模型。高脂饲料喂养14周时,将高脂饲料喂养实验兔随机分为颈椎旋转手法组和模型组,各10只。颈椎旋转手法组兔施行颈椎旋转手法,左右各旋转1次(手法以兔子颈椎旋转至极限即止),1次/3 d,共5次。结果与结论:对照组兔拉曼光谱图上未见明显的1 450 cm-1及1 660 cm-1脂质特征峰,颈椎旋转手法组和模型组兔动脉粥样硬化斑块均有明显的1 450 cm-1及1 660 cm-1脂质特征峰,但颈椎旋转手法组和模型组特征峰的相对强度差异无显著性意义(P>0.05)。说明在颈动脉粥样硬化病变早期,短期内施用颈椎旋转手法并不会增加模型兔颈动脉粥样硬化斑块的脂质含量。BACKGROUND： It is generally recognized that cervical rotation manipulation can increase the risk of detachment of unstable plaques in carotid atherosclerosis, but few studies are reported on the influence of cervical rotation manipulation on the stable plaque in early carotid atherosclerosis. OBJECTIVE： To explore the influence of the cervical rotation manipulation on the lipid contents in carotid atherosclerotic plaque in a rabbit model of early carotid atherosclerosis. METHODS： After being fed for 15 days with normal diet, 30 male New Zealand White rabbits were further fed for 18 weeks with normal diet（n = 10; control group） or a high-fat diet containing 2% cholesterol, 10% lard and 88% normal granules to build rabbit models of early carotid atherosclerosis with stable carotid plaque（n = 20）. At 14 weeks of feeding with high-fat diet, the experimental rabbits fed with high-fat diet were randomly divided into a cervical rotation manipulation group（n = 10） and a model group（n = 10）. The rabbits in the cervical rotation manipulation group underwent cervical rotation manipulation to the left and right sides, once each side, with the maximal range of rotation. Total five cervical rotation manipulations, once every 3 days, were performed. RESULETS AND CONCLUSION： The 1 450 cm^-1 and 1 660 cm^-1 peaks of the Raman spectrum of lipid in the carotid atherosclerotic plaque of rabbit models were not distinctly present in the control group, however, theywere obviously observed in the cervical rotation manipulation group and model group. Nevertheless, the relative intensity differences at spectrum characteristic peaks were not significant between cervical rotation manipulation group and model group（P〉 0.05）. The environmental findings indicate that the lipid content in carotid atherosclerotic plaque of rabbit models of early carotid atherosclerosis cannot be increased after short-term administration of cervical rotation manipulation.

关 键 词：推拿 脊柱 脉粥样硬化 光谱分析 拉曼 脂质沉积 实验动物 心肺及血管损伤动物模型 颈部推拿 颈椎旋转手法 动脉粥样硬化 早期病变 稳定斑块 拉曼光谱 稳定性 脂质含量 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]