脾脏^(18)F-FDG摄取在急性放射病中的诊断价值  

Diagnostic value of ^(18)F-FDG uptake by spleen in acute radiation disease

作　　者：吴少杰[1] 王玉珏[2] 郭坤元[1] 

机构地区：[1]南方医科大学珠江医院血液科,广州510282 [2]南方医科大学动物中心,广州510515

出　　处：《解放军医学杂志》2015年第7期554-558,共5页Medical Journal of Chinese People's Liberation Army

基　　金：广东省211工程三期重点学科建设项目(C1030380);南方医科大学博士启动项目(B1012041)~~

摘　　要：目的探讨18F-FDG摄取值是否可作为评估核事故后个体受照射剂量的快速精确测定方法。方法将48头西藏小型猪随机分为6个剂量组,分别为0、1、2、5、8、11Gy组。除0Gy组(对照组)外,其余组均接受全身8MV X线离心线性加速器照射。照射前与照射后6、24、72h实施全身18F-FDG PET/CT检查,收集脾脏组织用于组织病理学与凋亡检测;收集血液标本进行外周血分析。结果实验组与对照组的脾脏18F-FDG摄取值差异有统计学意义(P<0.05或P<0.01)。照射后6h,脾脏18F-FDG摄取值与照射剂量存在显著相关性,相关系数为0.95(P<0.01)。组织病理学观察显示脾脏组织损伤程度与所接受的放射剂量呈正比。流式细胞学结果表明脾脏淋巴细胞损伤以凋亡为主。结论在西藏小型猪放射线损伤模型中,脾脏18F-FDG摄取与照射剂量密切相关,18F-FDG PET/CT可用于急性放射病后个体照射剂量的快速评估。Objective To investigate whether 18F-FDG uptake can be applied in dosimetry to facilitate a rapid and accurate evaluation of individual radiation dosage after a nuclear accident. Methods Forty-eight Tibetan minipigs were randomly assigned into 6 groups, i.e., 0, 1, 2, 5, 8 and 11 Gy groups. Animals in all except 0Gy group received total body irradiation（TBI） with a 8MV X centrifugal linear accelerator, and 18F-FDG combined positron-emission tomography and computed tomography（PET/CT） were carried out before TBI, and also at 6, 24 and 72 h after receiving TBI in different doses ranging from 1 to 11 Gy. Spleen tissues and blood samples were collected for histological examination, apoptosis, and routine blood analysis. Results Mean standardized uptake values（SUVs） of the spleen showed significant differences between experimental groups and control group. The spleen SUVs at 6h post-irradiation showed significant correlation with radiation dose; Spearman＇s correlation coefficient was 0.95（P〈0.01）. Histopathological observations showed that the degree of splenic damage was proportional to the radiation dose. Moreover, flow cytometry revealed that apoptosis was one of the major forms of splenic lymphocyte death. Conclusion In the Tibetan minipig model, it was shown that radiation doses bear a close relationship with the 18F-FDG uptake of spleen. This finding suggests that 18F-FDG PET/CT may be useful for the rapid detection of individual radiation dosage after acute radiation disease（ARD）.

关 键 词：氟脱氧葡萄糖F18 正电子发射断层扫描及电脑断层扫描 脾 辐射剂量 急性辐射综合征 

分 类 号：R818.04[医药卫生—放射医学]