低剂量电离辐射、一氧化碳、苯和噪声复合作用下大鼠血清的代谢组学研究  被引量:4

Study on serum metabonomics of rats exposed to low-dose ionizing radiation, carbon monoxide, benzene and noise

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作  者:王庆蓉[1] 沈先荣[1] 何颖[1] 刘玉明[1] 李珂娴[1] 陈伟[1] 蒋定文[1] 侯登勇[1] 

机构地区:[1]海军医学研究所,上海200433

出  处:《解放军医学杂志》2015年第7期559-563,共5页Medical Journal of Chinese People's Liberation Army

基  金:全军后勤科研计划项目(AHJ09J012);总后科技攻关项目(2009183006)~~

摘  要:目的探讨低剂量电离辐射、一氧化碳、苯和噪声有害环境因素复合作用对大鼠血清代谢产物的影响,探讨复合环境因素导致机体损伤的机制。方法健康成年SD大鼠16只,随机分为正常对照组(不做任何处理)和实验组(每日接受低剂量电离辐射、一氧化碳、苯和噪声的复合作用,共7d),每组8只。实验结束时腹主动脉取血,分离大鼠血清,采用核磁共振氢谱(1H NMR)仪检测两组大鼠血清样本,得到代谢指纹图谱,采用主成分分析(PCA)和正交信号校正-偏最小二乘(OSC-PLS)法进行模式识别,并通过SIMCA-P软件鉴别两组代谢产物谱的差异。结果大鼠血清1H NMR图谱分析显示对照组和实验组的代谢谱存在明显差异,OSC-PLS分析显示两组代谢产物各自聚集,分布区域明显分开。与对照组比较,实验组血清中脂类、高密度脂蛋白、甘氨酸/葡萄糖、N-乙酰糖蛋白1、N-乙酰糖蛋白2、磷脂酰胆碱和不饱和脂肪酸含量增加,乳酸、苏氨酸/脂质、丙氨酸、肌酸、甘磷酸胆碱/氧化三甲胺、低密度脂蛋白/高密度脂蛋白、低密度脂蛋白、极低密度脂蛋白/低密度脂蛋白、极低密度脂蛋白和饱和脂肪酸含量下降。结论低剂量电离辐射、一氧化碳、苯和噪声有害环境因素复合作用使大鼠血清代谢产物发生变化,主要涉及免疫功能、肾功能和能量代谢。基于核磁共振的代谢组学方法可用于研究多种有害环境因素的复合生物效应,具有较好的应用前景。Objective To investigate the combined effects of low-dose ionizing radiation, carbon monoxide, benzene and noise on serum metabolites and the mechanism of injury induced by these complex environmental factors in rats. Methods Sixteen adult SD rats were randomly divided into control group and exposed group(8 each). The exposed group received the combined effect every day for 7 days. At the end of experiment, sera were collected from the abdominal aorta of rats. The metabolic fingerprint of serum was obtained by 1H nuclear magnetic resonance(1H NMR) spectroscopy and determined with pattern recognition techniques of principal component analysis(PCA) and orthogonal signal correction-partial least squares(OSC-PLS). The similarities and differences in metabolic profiles between two groups were visualized by SIMCA-P software. Results The rat serum 1H NMR spectra revealed different metabolic spectra between the control group and exposed group. The OSC-PLS plots of the serum samples presented respectively marked clustering between the two groups. Compared with the control group, the contents of lipid, high density lipoprotein, glycine/glucose, N-acetyl glycoprotein 1, N-acetyl glycoprotein 2, phosphatidyl choline and unsaturated fatty acid increased, while those of lactic acid, threonine/lipid, alanine, creatine, glycer ylphosphor ylcholine/ trimethylamine oxide, low density lipoprotein/high density lipoprotein, low density lipoprotein, very low density lipoprotein/low density lipoprotein, very low density lipoprotein and saturated fatty acid decreased. Conclusions Combination of low-dose ionizing radiation, carbon monoxide, benzene and noise could induce changes of serum metabolites in rats, involving in immune function, renal function and energy metabolism. The NMR-based-metabonomics method has potential of application in research on combined biological effects of the complex environmental factors.

关 键 词:辐射损伤 一氧化碳  噪声 代谢组学 

分 类 号:R148[医药卫生—公共卫生与预防医学] R135

 

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