羟基红花黄色素通过RhoA/ROCK通路保护大鼠心肌再灌注损伤的机制研究  

Mechanism of hydroxysafflor yellow protect myocardial ischemia reperfusion through the signaling pathway of RhoA/ROCK in rats

作　　者：王亚光[1] 王鹏[2] 刘洪光[1] 张媛媛[1] 陶贵周[1] 黄建华[2] 

机构地区：[1]辽宁医学院附属第一医院心内科,辽宁锦州121001 [2]辽宁医学院附属第一医院组织工程重点实验室

出　　处：《临床心血管病杂志》2015年第7期791-794,共4页Journal of Clinical Cardiology

摘　　要：目的:研究羟基红花黄色素A对大鼠离体缺血心脏再灌注的保护作用,探讨其与Rho/ROCK信号通路的作用。方法:36只大鼠被随机分为7组,采用离体心脏灌流模型,给予缺血预处理及药物灌流,比较各组心率、冠状动脉流量,测定左心室内压,TTC心肌染色评估梗死范围,Western blot检测心肌组织内ROCK1、ROCK2活性以及Caspase-3的表达。结果:治疗组大鼠心脏缺血再灌注损伤减轻,心肌梗死面积减少,ROCK1、ROCK2活性以及Caspase-3的表达减低。结论:羟基红花黄色素A能够减轻大鼠心脏缺血再灌注损伤,同时减少心肌梗死面积,其作用机制可能与其抑制RhoA/ROCK信号通路,减少Caspase-3的表达有关。Objective：To investigate the effects of hydroxysafflor yellow on rats＇ myocardial ischemia reperfusion and explore the relationship between hydroxysafflor yellow and the access of RhoA/ROCK.Method：Thirtysix rats were randomly divided into 7groups and made isolated heart perfusion model with the treatment of medical reperfusion.HR,CF,LV＋dp/dtmax,LV-dp/dtmax,LVDP were detected.The myocardial infarct size was detected by TTC,and the density of ROCK1,ROCK2 and Caspase-3were detected by Western blot.Result：The damage of myocardial ischemia reperfusion injury and the expression of ROCK1,ROCK2 and Caspase-3were reduced in treatment group.Conclusion：Hydroxysafflor yellow can protect the myocardial ischemia reperfusion injury in rats,and also reduce the infarct size.The mechanism may be related to inhibition of the signalling pathway of RhoA/ROCK,and the reduction of Caspase-3expression.

关 键 词：羟基红花黄色素A 缺血再灌注损伤 RhoA/ROCK CASPASE-3 

分 类 号：R965.2[医药卫生—药理学]