2型糖尿病神经病理性痛C57BL/6J小鼠模型的建立  

Establishment of the model of type 2 diabetic neuropathic pain in C57BL/6J mice

作　　者：杨振玲[1] 乔长峰[1] 张学良[1] 徐高磊[2] 陈文武[3] 

机构地区：[1]河南大学第一附属医院疼痛科 [2]郑州大学基础医学院解剖学教研室,河南郑州450000 [3]河南大学第一附属医院神经内科,河南开封475001

出　　处：《解剖科学进展》2015年第4期345-348,共4页Progress of Anatomical Sciences

基　　金：河南大学科研基金项目(2012YBZR0221)

摘　　要：目的利用胃饲酒精和腹腔注射链脲佐菌素(streptozotocin,STZ)建立2型糖尿病神经病理性痛C57BL/6J小鼠模型。方法 80只C57BL/6J小鼠随机分为:对照组(n=15)和实验组(n=65)。对照组每日胃饲生理盐水,12周后单次空腹腹腔注射柠檬酸缓冲液;实验组每日胃饲酒精12周诱导胰岛素抵抗,继以不同剂量链脲佐菌素(40 mg/kg、50 mg/kg、60 mg/kg)腹腔注射1次,于不同时间点分别测体重、血糖、血胰岛素浓度,计算胰岛素抵抗指数、机械缩足阈值和热缩足潜伏期的变化。结果连续胃饲酒精12周后,实验组小鼠体重明显增加,空腹胰岛素浓度升高,胰岛素抵抗指数升高,血糖未升高,机械缩足阈值和热缩足潜伏期无变化。注射STZ后,40 mg/kg剂量组血糖升高但不能长期维持,60 mg/kg剂量组血糖较高,死亡率高;50 mg/kg剂量组血糖中度升高且相对稳定,胰岛素浓度和胰岛素敏感性均降低,其机械缩足阈值和热缩足潜伏期均低于基础值和对照组(P<0.05)。结论连续酒精胃饲12周后联合腹腔注射STZ50 mg/kg可以建立理想的2型糖尿病神经病理性痛小鼠模型。Objective To establish the model of type 2 diabetic neuropathic pain in C57BL/6J mice by alcohol exposure and STZ injection. Methods 80 C57BL/6J mice were randomly divided into control group（C-group, n=15） and experiment group（E-group, n=65）. The mice in C-group were fed with normal diet for 12 weeks, and then injected intraperitoneally with citrate buffer. The mice in E-group were fed with alcohol for 12 weeks to induce insulin resistance, and then injected intraperitoneally with different doses of streptozotocin（STZ, 40mg/kg, 50mg/kg, 60mg/kg）. The values of body weight, fasting plasma glueose（FPG）, fasting plasma insulin（FINS） and insulin resistance index（IRI）, mechanical withdrawal threshold（MWT） and thermal withdrawal latcncy（TWL） were determined at different time points before and after injecting STZ respectively. Results The values of body weight, serum insulin level and IRI were increased significantly in model mice than in control mice, with no changes for mechanical and thermal withdrawal threshold 12 weeks after alcohol feeding. After STZ injection, hyperglycemia developed in mice （40mg/kg） but not maintaining a long period, higher blood glucose and highest mortality were found in mice（60 mg/kg）; however, in mice（50mg/kg）, stable moderate hyperglycemia appeared with lower serum insulin level and insulin sensitivity, and lower mechanical withdrawal threshold and thermal withdrawal threshold compared with basal level and control group（P〈0.05）. Conclusion Alcohol feeding for 12 weeks combined with low dose STZ （50mg/kg） could induce an ideal type 2 diabetic neuropathic pain in mice.

关 键 词：酒精 胰岛素抵抗 2型糖尿病 链脲佐菌素 神经病理性痛 C57BL/6J小鼠 

分 类 号：R587.1[医药卫生—内分泌]