拟南芥诱导型启动子rd29A的克隆及其功能鉴定  

Cloning and functional characterization of an inducible promoter rd29A from Arabidopsis thaliana

作　　者：齐恩芳[1,2,3] 贾小霞[1,2,3] 马胜[1,2,3] 文国宏[1,2,3] 胡新元[1,2,3] 龚成文[1,2,3] 王一航[1,2,3] 李建武[1,2,3] 

机构地区：[1]甘肃省农业科学院马铃薯研究所,甘肃兰州730070 [2]甘肃省马铃薯种质资源创新工程实验室,甘肃兰州730070 [3]农业部西北旱作马铃薯科学观测实验站,甘肃渭源748201

出　　处：《干旱地区农业研究》2015年第4期286-290,共5页Agricultural Research in the Arid Areas

基　　金：甘肃省农业科学院中青年基金项目(2014GAAS20);国家自然科学基金(31060200;31160299;31360353)

摘　　要：以改善作物抗旱性为目的,采用PCR方法从拟南芥中克隆了诱导型启动子rd29A,序列分析发现克隆的rd29A启动子与已发表的rd29A启动子序列(D13044)的同源性为99.47%。利用DNA重组技术成功构建了rd29A启动子驱动GUS基因的植物表达载体p BI121-rd29-GUS,并通过农杆菌介导法转化烟草,转基因烟草叶片中GUS酶活性的组织化学检测结果表明,rd29A启动子能驱动目的基因的有效表达。因此,可以在后续的马铃薯抗旱转基因研究中直接应用。To improve drought resistance of crops,inducible promoter rd29 A was cloned from Arabidopsis thaliana by PCR method. Sequencing analysis indicated that the cloned fragment showed 99. 47% identity to the published sequence（ D13044）. An rd29 a driving GUS expression vector p BI121- rd29- GUS was constructed by DNA recombinant technology. By Agrobacterium-mediated transformation,p BI121- rd29- GUS was transformed into tobacco. The function of rd29 A promoter was identified through the expression of GUS protein in transgenic tobacco. GUS activity in transgenic tobacco leaf showed that the rd29 A promoter could drive efficient expression of the target gene. rd29 A promoter could be used in subsequent transgenic study of drought resistance in potato.

关 键 词：RD29A启动子 抗逆性 GUS基因 

分 类 号：S532.03[农业科学—作物学] Q943.2[生物学—植物学]