活菌制剂冻干保护剂的研究进展  

Advances on cryoprotectants of live bacteria preparations

作　　者：张光磊[1] 张新创[1] 翟雷[1] 

机构地区：[1]兰州生物制品研究所有限责任公司甘肃省疫苗工程技术研究中心,甘肃兰州730046

出　　处：《微生物学免疫学进展》2015年第4期80-85,共6页Progress In Microbiology and Immunology

摘　　要：冷冻干燥技术广泛应用于细菌性活疫苗生产和菌种保藏等方面。细菌细胞在冷冻干燥过程中会出现损伤,甚至死亡。通过添加适合的冻干保护剂可以最大程度减小细胞损伤,保持活菌制剂的活力和性能。就冻干过程对细菌的损伤机制、冻干保护剂的作用机理以及保护剂的筛选方法等方面进行了阐述,对活菌制剂保护剂的筛选有所启示。The freeze-drying technology is widely used for the production of live bacterial vaccine and culture preservation.The bacterial cells suffering from the freeze-drying will be damaged,and even death. Adding suitable cryoprotectants can reduce the damage of cells to the maximum extent,improving the viability and performance of live bacteria preparations. In this paper,the mechanism of freeze-drying injury to bacteria,the protective effect of cryoprotectants and the methods screening protective agents are systematically described,hoping to gain some inspirations in screening protectants of live bacteria preparations.

关 键 词：活菌制剂 冷冻干燥 冻干保护剂 

分 类 号：R392[医药卫生—免疫学]