罗格列酮对2型糖尿病大鼠胰腺PDX-1基因表达的影响  

Effect of rosiglitazone on pancreatic PDX-1 gene expression in rats with type 2 diabetes mellitus

作　　者：陈晓铭[1] 郑坤杰[1] 吴美芬[1] 方烁[1] 胡卓清 李潍[1] 刘美莲[1] 武革[1] 

机构地区：[1]广东医学院附属医院内分泌科,广东湛江524001

出　　处：《广东医学院学报》2015年第2期144-146,150,共4页Journal of Guangdong Medical College

基　　金：广东省科技计划项目(No.2011B031800231);广东省医学科研基金立项课题(No.B2011243);湛江市非资助科技攻关计划项目(No.2013B01099)

摘　　要：目的观察罗格列酮(RSG)对2型糖尿病(T2DM)大鼠胰岛β细胞功能、胰岛细胞增殖以及胰腺十二指肠同源盒-1(PDX-1)基因表达的影响。方法将36只SD雄性大鼠随机分为正常对照组、T2DM未治疗组和RSG组。T2DM未治疗组和RSG组给予高脂饲料喂养,于第8周末以链脲佐霉素(STZ)30 mg/kg腹腔注射,成模后RSG组每天给予RSG 4mg/kg灌胃持续8周。检测各组空腹血糖以及胰岛素水平;12周后处死大鼠立即取出胰腺组织,用免疫组化染色和荧光定量PCR观察胰岛增殖细胞核抗原(PCNA)、PDX-1 m RNA表达。结果与正常对照组相比,T2DM未治疗组PDX-1m RNA表达减少(P<0.01);与T2DM未治疗组比较,RSG组大鼠PCNA和PDX-1m RNA基因表达增加(P<0.01)。结论RSG上调T2DM大鼠胰腺PDX-1基因表达,改善胰岛β细胞功能。Objective To observe the effect of rosiglitazone（RSG） on the pancreatic beta cell function and proliferation and pancreatic duodenal homeobox-1（PDX-1） gene expression in rats with type 2 diabetes mellitus（T2DM）. Methods Thirty-six SD rats were randomly divided into normal control, T2 DM and RSG groups. T2 DM and RSG groups were intraperitoneally injected with 30 mg/kg streptozocin after feeding with high-fat diet for 8 weeks. The RSG group was treated with daily intragastric RSG（4 mg/kg） for 8 weeks. Levels of fasting blood glucose and insulin were detected. All rats were sacrificed and pancreases were removed after 12 weeks. Proliferating cell nuclear antigen（PCNA） of pancreatic islets and PDX-1 m RNA were detected by immunohistochemistry and fluorescent quantitative PCR, respectively. Results Expression of PDX-1 m RNA was significantly lower in T2 DM group than that in normal control group（P〈0.01）. Compared with the T2 DM group, expression of PCNA and PDX-1 m RNA was increased in RSG group（P〈0.01）. Conclusion RSG can upregulate the expression of PDX-1 m RNA and improve the function of beta cells.

关 键 词：糖尿病 大鼠 PDX-1 罗格列酮 

分 类 号：R587.1[医药卫生—内分泌]