淫羊藿苷干预ConA诱导体外BRL-3A细胞损伤的机制  

Effect of icariin on Con A-induced BRL-3A cell injury in vitro

作　　者：曹怡[1] 刘波[1] 徐彭[1] 周年[1] 陈志[1] 候阳阳 

机构地区：[1]江西中医药大学药学院,南昌330004

出　　处：《中药药理与临床》2015年第3期21-24,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金项目(NO:81160389);江西省科技计划项目(NO:20122BBG70090-1);江西省自然科学基金项目(NO:20114BAB205073)

摘　　要：目的:研究淫羊藿主要成分淫羊藿苷对ConA(刀豆蛋白)致体外BRL-3A细胞(大鼠肝细胞)损伤的影响和作用机制。方法:体外培养BRL-3A细胞,分别加入25、50、100μmol/L的淫羊藿苷24h,再以100μg/ml的ConA诱导损伤24h,MTT法检测BRL-3A细胞的存活率,考察细胞上清液中谷草转氨酶、谷丙转氨酶、超氧化物歧化酶、丙二醛、谷胱甘肽过氧化物酶含量,以荧光探针DCFH-DA检测细胞内活性氧含量,免疫细胞化学方法检测肝细胞Bax、Bcl-2表达水平。结果:淫羊藿苷显著提高ConA刺激后BRL-3A的存活率,25μmol/L、50μmol/L、100μmol/L淫羊藿苷均明显降低谷草转氨酶的含量;100μmol/L淫羊藿苷明显降低谷丙转氨酶的含量,并明显升高超氧化物歧化酶、谷胱甘肽过氧化物酶的活性,降低丙二醛、活性氧含量,增强Bcl-2的表达,降低Bax的表达。结论:淫羊藿苷能明显改善ConA诱导的BRL-3A细胞损伤,作用机制与淫羊藿苷抗氧化相关。Objective： To investigate the effect and the underlying mechanism of icariin on ConA-indueed hepatic injury. Methods： The rat hepato- cytes （BRL-3A） were cultured with icariin（25,50 and 100μmol/L） for 24h, and then the ConA（100μg/ml） were added for 24h. The cell viability was measured by MTT assay, the activities of AST, ALT and the levels of SOD, MDA, GSH-Px in supematant were determined, the levels of intraceilular ROS was measured with DCFH-DA. Results： The results showed that ConA induced-the survival rate of BRL-3A and the activity of SOD and GSH-Px were significantly improved by icariin（25 ~ 100 ~mol/L）. And the release of AST and ALT and the content of MDA, ROS were also deereasedby icafiin（ 100 Ixmol/L）. In the immunocytochemistry, the expression of Bax was decreased by ieariin, and the expression of Bcl-2 increased. Conclusion： These results indicated that icariin protect BRL-3A cells from ConA-induced cytotoxicity, and the anti-oxidative stress may be one of mechanisms.

关 键 词：淫羊藿苷 CONA 氧化应激 

分 类 号：R285[医药卫生—中药学]